| Transcriptional regulation of plasminogen activator inhibitor type-1 mRNA in Hep G2 cells by epidermal growth factor. | |
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MedLine Citation:
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PMID: 2011496 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Secretion of plasminogen activator inhibitor type-1 (PAI-1) by cultured cells is increased after exposure to specific cytokines and growth factors. We have shown previously that incubation of Hep G2 cells with epidermal growth factor (EGF) results in a marked increase in steady state levels of PAI-1 mRNA (Lucore, C.L., et al. (1988) J. Biol. Chem. 263, 15845-15848). The present study was undertaken to determine whether the regulation of expression of PAI-1 mRNA by EGF is mediated at the level of transcription and/or by post-transcriptional mechanisms. The rate of transcription of the PAI-1 gene measured by nuclear run-on assays was found to be increased within 2 h after stimulation of the cells with EGF (5 ng/ml) (3.2 fold increase relative to control, n = 2, range 3.0-3.4). It reached a maximum in 3 h, (9.2 fold increase relative to control, n = 2, range 8.8-9.6) and returned to baseline in 5 h. Exposure of the cells to EGF did not increase the rate of transcription of the glyceraldehyde-3-phosphate dehydrogenase gene. The half life of PAI-1 mRNA in Hep G2 cells was 120 min as determined by RNA blot analysis after exposure of the cells to actinomycin D to inhibit transcription. Stimulation of the cells with EGF did not result in significant change in the half life of PAI-1 mRNA. The results demonstrate that exposure of Hep G2 cells to EGF increases PAI-1 gene transcription. |
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Authors:
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W E Hopkins; D R Westerhausen; B E Sobel; J J Billadello |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Nucleic acids research Volume: 19 ISSN: 0305-1048 ISO Abbreviation: Nucleic Acids Res. Publication Date: 1991 Jan |
Date Detail:
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Created Date: 1991-05-07 Completed Date: 1991-05-07 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0411011 Medline TA: Nucleic Acids Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 163-8 Citation Subset: IM |
Affiliation:
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Department of Medicine, Washington University School of Medicine, St Louis, MO 63110. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Cycloheximide
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pharmacology Epidermal Growth Factor / physiology* Gene Expression Regulation Glyceraldehyde-3-Phosphate Dehydrogenases / genetics, metabolism Half-Life Humans Kinetics Plasminogen Inactivators / metabolism* RNA, Messenger / biosynthesis, metabolism Transcription, Genetic* Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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HL 17646/HL/NHLBI NIH HHS; HL 38868/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Plasminogen Inactivators; 0/RNA, Messenger; 62229-50-9/Epidermal Growth Factor; 66-81-9/Cycloheximide; EC 1.2.1.-/Glyceraldehyde-3-Phosphate Dehydrogenases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): Nucleic Acids Res ISSN: 0305-1048 ISSN: 1362-4962 |
Article Information Download PDF  Print publication date: Day: 11 Month: 1 Year: 1991 Volume: 19 Issue: 1 First Page: 163 Last Page: 168 ID: 333547 PubMed Id: 2011496 |
| Transcriptional regulation of plasminogen activator inhibitor type-1 mRNA in Hep G2 cells by epidermal growth factor. | |
| W E Hopkins | |
| D R Westerhausen, Jr | |
| B E Sobel | |
| J J Billadello | |
| Department of Medicine, Washington University School of Medicine, St Louis, MO 63110. |
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