| Transcriptional regulation of pilC2 in Neisseria gonorrhoeae: response to oxygen availability and evidence for growth-phase regulation in Escherichia coli. | |
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MedLine Citation:
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PMID: 9268019 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The type-4 pilus of Neisseria gonorrhoeae is a dominant surface antigen which facilitates adhesion to host target cells, an essential event in gonococcal infection. pilC2 encodes a 110-kDa protein involved in pilus assembly, pilus-mediated adherence to human epithelial cells in culture and natural competence for DNA transformation. Luciferase activity directed from a chromosomal pilC2::luxAB transcriptional fusion was reduced approximately 4-fold when cells were grown anaerobically. We observed a concomitant reduction in gonococcal piliation by electron microscopy and a reduction in the ability to adhere to ME-180 human epithelial cells when bacteria were grown in the absence of oxygen. Furthermore, we present evidence for growth-phase regulation of the gonococcal pilC2 gene in Escherichia coli, and show that all sequences necessary for growth-phase regulation are contained on a 121-bp pilC2 fragment. Expression from the minimal pilC2 fragment fused to lacZ in single-copy in E. coli was induced 2-fold when cells entered stationary phase. Surprisingly, induction does not require rpoS, the gene, which encodes the starvation-induced sigma factor RpoS. In summary, we have demonstrated that pilC2 is both positively and negatively regulated at the level of transcription. This regulation is most probably relevant to physiological conditions within the human host which influence gonococcal infections. |
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Authors:
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J Mellies; T Rudel; T F Meyer |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular & general genetics : MGG Volume: 255 ISSN: 0026-8925 ISO Abbreviation: Mol. Gen. Genet. Publication Date: 1997 Jul |
Date Detail:
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Created Date: 1997-09-18 Completed Date: 1997-09-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0125036 Medline TA: Mol Gen Genet Country: GERMANY |
Other Details:
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Languages: eng Pagination: 285-93 Citation Subset: IM |
Affiliation:
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Max-Planck-Institut für Biologie, Abteilung Infektionsbiologie, Tübingen, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anaerobiosis Bacterial Adhesion / genetics Bacterial Proteins / biosynthesis*, genetics Base Sequence Cell Division Cells, Cultured Escherichia coli / drug effects, genetics*, pathogenicity Fimbriae Proteins* Fimbriae, Bacterial / genetics, ultrastructure Gene Expression Regulation, Bacterial* Humans Molecular Sequence Data Neisseria gonorrhoeae / drug effects, genetics*, pathogenicity, ultrastructure Oxygen / pharmacology* Transcription, Genetic Virulence / genetics |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/pilC protein, Neisseria gonorrhoeae; 147680-16-8/Fimbriae Proteins; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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