| Transcriptional regulation of a new variant of human platelet-derived growth factor receptor alpha transcript by E2F-1. | |
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MedLine Citation:
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PMID: 17881156 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Platelet-derived growth factors (PDGFs) and their receptors play an important role in cell proliferation, angiogenesis, and differentiation during normal development, and have also been implicated in tumorigenesis. In this study, we identified a novel variant of human PDGF receptor alpha mRNA (type II), which contains the same open reading frame as the known PDGF receptor alpha mRNA (type I) but a different 5'-untranslated region (5'-UTR). The 5'-UTR of the type II transcript was identified as a 363-bp exon located in intron 1 at position +1,210 to +1,572 relative to the transcriptional initiation site of the type I transcript. This type II transcript was expressed in a subset of human cell lines, such as MG-63 and MNNG/HOS cells. Moreover, transcription of the type II, but not the type I, was regulated by E2F-1 through an E2F-1-responsive site located at position +1,086/+1,093 downstream of the transcriptional initiation site of the type I transcript. Furthermore, epigenetic modulation might be involved in the expression of the type II transcript. Our findings provide new insights into the regulatory mechanism of PDGF receptor alpha transcription in normal and tumor cells. |
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Authors:
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Yusuke Minato; Etsu Tashiro; Mai Kanai; Yoshiaki Nihei; Yuki Kodama; Masaya Imoto |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-08-25 |
Journal Detail:
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Title: Gene Volume: 403 ISSN: 0378-1119 ISO Abbreviation: Gene Publication Date: 2007 Nov |
Date Detail:
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Created Date: 2007-10-05 Completed Date: 2007-11-27 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 7706761 Medline TA: Gene Country: Netherlands |
Other Details:
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Languages: eng Pagination: 89-97 Citation Subset: IM |
Affiliation:
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Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University, Yokohama 223-8522, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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5' Untranslated Regions Base Pairing Cell Line Cell Line, Tumor DNA Primers E2F1 Transcription Factor / genetics, metabolism* Epigenesis, Genetic Exons Gene Expression Regulation Genes, Reporter Genetic Variation* Humans Introns Kidney / cytology Luciferases / metabolism Models, Genetic Open Reading Frames Osteosarcoma / pathology Plasmids RNA, Messenger / genetics Receptor, Platelet-Derived Growth Factor alpha / genetics* Reverse Transcriptase Polymerase Chain Reaction Transcription Initiation Site Transcription, Genetic* |
| Chemical | |
Reg. No./Substance:
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0/5' Untranslated Regions; 0/DNA Primers; 0/E2F1 Transcription Factor; 0/RNA, Messenger; EC 1.13.12.-/Luciferases; EC 2.7.10.1/Receptor, Platelet-Derived Growth Factor alpha |
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