Document Detail

Transcriptional regulation of the mouse interleukin-2 receptor beta chain gene by Ets and Egr-1.
MedLine Citation:
PMID:  15752766     Owner:  NLM     Status:  MEDLINE    
To clarify the mechanisms and factors involved in the regulation of mouse IL-2Rbeta gene expression, we isolated the 5'-flanking region of IL-2Rbeta gene and investigated the promoter activity. Here we elucidated the positive regulatory regions, the most potent of which are located between -50 to -30bp and -164 to -135bp. These regions contain a potentially functional Ets and Egr-1-binding sites whose mutations abrogate promoter activity. Data from electrophoretic mobility shift assay indicate that Ets and Egr-1, but not Sp1, bind to the positive regulatory regions, -50 to -30bp and -164 to -135bp, respectively. Furthermore, recruitment of Ets and Egr-1 at endogenous IL-2Rbeta promoter segments in an IL-2-dependent F7 cells was verified by the chromatin immunoprecipitation assay. This study for the first time delineates the molecular mechanisms underlying regulation of mouse IL-2Rbeta gene transcription by Ets family proteins, partially with Egr-1, and thereby further elucidates the molecular basis of lymphocyte activation and differentiation.
Sang-Kyu Ye; Tack Joong Kim; Sung Sik Won; Taek Joon Yoon; Tae Kyu Park; Yung Choon Yoo; Yong-Nyun Kim; Hai Chon Lee; Koichi Ikuta; Myung-Hee Chung; Kwang Ho Lee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  329     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-03-08     Completed Date:  2005-05-16     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1094-101     Citation Subset:  IM    
Department of Pharmacology, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul 110-799, Republic of Korea.
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MeSH Terms
Cell Line
DNA-Binding Proteins / metabolism*
Early Growth Response Protein 1
Gene Expression Regulation / physiology*
Immediate-Early Proteins / metabolism*
Interleukin-2 Receptor beta Subunit
Promoter Regions, Genetic
Proto-Oncogene Proteins / metabolism*
Proto-Oncogene Proteins c-ets
Receptors, Interleukin / metabolism*
Transcription Factors / metabolism*
Transcriptional Activation / physiology*
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Early Growth Response Protein 1; 0/Egr1 protein, mouse; 0/Il2rb protein, mouse; 0/Immediate-Early Proteins; 0/Interleukin-2 Receptor beta Subunit; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-ets; 0/Receptors, Interleukin; 0/Transcription Factors

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