| Transcriptional regulation of the mouse interleukin-2 receptor beta chain gene by Ets and Egr-1. | |
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MedLine Citation:
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PMID: 15752766 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To clarify the mechanisms and factors involved in the regulation of mouse IL-2Rbeta gene expression, we isolated the 5'-flanking region of IL-2Rbeta gene and investigated the promoter activity. Here we elucidated the positive regulatory regions, the most potent of which are located between -50 to -30bp and -164 to -135bp. These regions contain a potentially functional Ets and Egr-1-binding sites whose mutations abrogate promoter activity. Data from electrophoretic mobility shift assay indicate that Ets and Egr-1, but not Sp1, bind to the positive regulatory regions, -50 to -30bp and -164 to -135bp, respectively. Furthermore, recruitment of Ets and Egr-1 at endogenous IL-2Rbeta promoter segments in an IL-2-dependent F7 cells was verified by the chromatin immunoprecipitation assay. This study for the first time delineates the molecular mechanisms underlying regulation of mouse IL-2Rbeta gene transcription by Ets family proteins, partially with Egr-1, and thereby further elucidates the molecular basis of lymphocyte activation and differentiation. |
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Authors:
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Sang-Kyu Ye; Tack Joong Kim; Sung Sik Won; Taek Joon Yoon; Tae Kyu Park; Yung Choon Yoo; Yong-Nyun Kim; Hai Chon Lee; Koichi Ikuta; Myung-Hee Chung; Kwang Ho Lee |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 329 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2005 Apr |
Date Detail:
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Created Date: 2005-03-08 Completed Date: 2005-05-16 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 1094-101 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul 110-799, Republic of Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line DNA-Binding Proteins / metabolism* Early Growth Response Protein 1 Gene Expression Regulation / physiology* Immediate-Early Proteins / metabolism* Interleukin-2 Receptor beta Subunit Mice Promoter Regions, Genetic Proto-Oncogene Proteins / metabolism* Proto-Oncogene Proteins c-ets Receptors, Interleukin / metabolism* Transcription Factors / metabolism* Transcriptional Activation / physiology* |
| Chemical | |
Reg. No./Substance:
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0/DNA-Binding Proteins; 0/Early Growth Response Protein 1; 0/Egr1 protein, mouse; 0/Il2rb protein, mouse; 0/Immediate-Early Proteins; 0/Interleukin-2 Receptor beta Subunit; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-ets; 0/Receptors, Interleukin; 0/Transcription Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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