| Transcriptional regulation of the gene encoding an alcohol dehydrogenase in the archaeon Sulfolobus solfataricus involves multiple factors and control elements. | |
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MedLine Citation:
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PMID: 12813087 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A transcriptionally active region has been identified in the 5' flanking region of the alcohol dehydrogenase gene of the crenarchaeon Sulfolobus solfataricus through the evaluation of the activity of putative transcriptional regulators and the role of the region upstream of the gene under specific metabolic circumstances. Electrophoretic mobility shift assays with crude extracts revealed protein complexes that most likely contain TATA box-associated factors. When the TATA element was deleted from the region, binding sites for both DNA binding proteins, such as the small chromatin structure-modeling Sso7d and Sso10b (Alba), and transcription factors, such as the repressor Lrs14, were revealed. To understand the molecular mechanisms underlying the substrate-induced expression of the adh gene, the promoter was analyzed for the presence of cis-acting elements recognized by specific transcription factors upon exposure of the cell to benzaldehyde. Progressive dissection of the identified promoter region restricted the analysis to a minimal responsive element (PAL) located immediately upstream of the transcription factor B-responsive element-TATA element, resembling typical bacterial regulatory sequences. A benzaldehyde-activated transcription factor (Bald) that specifically binds to the PAL cis-acting element was also identified. This protein was purified from heparin-fractionated extracts of benzaldehyde-induced cells and was shown to have a molecular mass of approximately 16 kDa. The correlation between S. solfataricus adh gene activation and benzaldehyde-inducible occupation of a specific DNA sequence in its promoter suggests that a molecular signaling mechanism is responsible for the switch of the aromatic aldehyde metabolism as a response to environmental changes. |
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Authors:
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Gabriella Fiorentino; Raffaele Cannio; Mosè Rossi; Simonetta Bartolucci |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of bacteriology Volume: 185 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 2003 Jul |
Date Detail:
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Created Date: 2003-06-18 Completed Date: 2003-07-10 Revised Date: 2013-04-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: United States |
Other Details:
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Languages: eng Pagination: 3926-34 Citation Subset: IM |
Affiliation:
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Dipartimento di Chimica Biologica, Università degli Studi di Napoli Federico II, Naples, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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5' Flanking Region
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genetics Alcohol Dehydrogenase / genetics, metabolism* Archaeal Proteins / genetics, metabolism* Base Sequence Benzaldehydes / metabolism Culture Media DNA Footprinting DNA, Archaeal / metabolism DNA-Binding Proteins / metabolism Gene Expression Regulation, Archaeal* Molecular Sequence Data Promoter Regions, Genetic Sulfolobus / enzymology*, growth & development TATA Box / genetics Transcription Factors / metabolism Transcription, Genetic* Transcriptional Activation |
| Chemical | |
Reg. No./Substance:
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0/Archaeal Proteins; 0/Benzaldehydes; 0/Culture Media; 0/DNA, Archaeal; 0/DNA-Binding Proteins; 0/Transcription Factors; EC 1.1.1.1/Alcohol Dehydrogenase; TA269SD04T/benzaldehyde |
| Comments/Corrections | |
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