Document Detail

Transcriptional regulation of Zic3 by heterodimeric AP-1(c-Jun/c-Fos) during Xenopus development.
MedLine Citation:
PMID:  15557819     Owner:  NLM     Status:  MEDLINE    
The heterodimeric c-Jun/c-Fos, an activator protein-1 (AP-1) has been implicated in mesoderm induction (Dong et al., 1996; Kim et al., 1998) whereas the homodimer of c-Jun was reported to be involved in neural inhibition during the early development of Xenopus embryos. During the early vertebrate development AP-1 involvement in the neural induction is still not clearly understood. We report here that AP-1 has a role in Zic3 expression, a critical proneural gene and a primary regulator of neural and neural crest development (Nakata et al., 1997; Nakata et al., 1998). AP-1 was able to induce the Zic3 gene in a dose dependent manner but other homo- or hetero-dimeric proteins, such as c-Jun/c-Jun, JunD/FosB or JunD/Fra-1 were not. The inhibition of AP-1 activity using morpholino antisenses of c-jun mRNAs blocked the Zic3 expression induced by activin. In addition, co-injection of c-jun mRNA rescued the down-regulated Zic3 expression. The promoter region of isolated Zic3 genomic DNA was found to possess several consensus-binding site of AP-1. Thus, in the functional assays, AP-1 could increase promoter activity of Zic3 gene. These findings suggest that proneural gene, Zic3 may be regulated by heterodimeric AP-1(c-Jun/c-Fos) and it may have a role in activin signaling for the regulation of neural specific gene, Zic3.
Sung-Young Lee; Hyun-Shik Lee; Jin-Soo Moon; Jong-Il Kim; Jae-Bong Park; Jae-Yong Lee; Mae Ja Park; Jaebong Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental & molecular medicine     Volume:  36     ISSN:  1226-3613     ISO Abbreviation:  Exp. Mol. Med.     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2004-11-23     Completed Date:  2005-04-26     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9607880     Medline TA:  Exp Mol Med     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  468-75     Citation Subset:  IM    
Department of Biochemistry, College of Medicine, Hallym University, Chuncheon, Kangwon-do 200-702, Korea.
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MeSH Terms
Activins / pharmacology,  physiology
Base Sequence
Binding Sites / genetics
Consensus Sequence / genetics
Embryo, Nonmammalian / metabolism
Gene Expression Regulation, Developmental*
Homeodomain Proteins / genetics*
Molecular Sequence Data
Promoter Regions, Genetic / genetics
Proto-Oncogene Proteins c-fos / genetics,  physiology
Proto-Oncogene Proteins c-jun / genetics,  physiology
RNA, Antisense / genetics
Transcription Factor AP-1 / genetics,  physiology*
Transcription Factors / genetics*
Transcription, Genetic*
Xenopus Proteins / genetics*
Xenopus laevis / embryology*,  genetics*
Reg. No./Substance:
0/Homeodomain Proteins; 0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-jun; 0/RNA, Antisense; 0/Transcription Factor AP-1; 0/Transcription Factors; 0/Xenopus Proteins; 0/Zic3 protein, Xenopus; 104625-48-1/Activins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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