Document Detail

Transcriptional regulation of the Schizosaccharomyces pombe gene encoding glutathione S-transferase I by a transcription factor Pap1.
MedLine Citation:
PMID:  15650694     Owner:  NLM     Status:  MEDLINE    
In a previous study, a gst gene was isolated from the fission yeast Schizosaccharomyces pombe. This gene was dubbed gst I, and was characterized using the gstI-lacZ fusion plasmid pYSH2000. In this work, four additional fusion plasmids, pYSHSD1, pYSHSD2, pYSHSD3 and pYSHSD4, were constructed, in order to carry (respectively) 770, 551, 358 and 151 bp upstream regions from the translational initiation point. The sequence responsible for induction by aluminum, mercury and hydrogen peroxide was located in the range between -1,088 and -770 bp upstream of the S. pombe gst I gene. The same region was identified to contain the nucleotide sequence responsible for regulation by Pap1, and has one putative Pap1 binding site, TTACGTAT, located in the range between -954 approximately -947 bp upstream of the gst I gene. Negatively acting sequences are located between -1,088 and -151 bp. These findings imply that the Pap1 protein is involved in basal and inducible transcription of the gst I gene in the fission yeast S. pombe.
Hong-Gyum Kim; Byung-Chul Kim; Kyunghoon Kim; Eun-Hee Park; Chang-Jin Lim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of microbiology (Seoul, Korea)     Volume:  42     ISSN:  1225-8873     ISO Abbreviation:  J. Microbiol.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2005-01-14     Completed Date:  2005-06-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9703165     Medline TA:  J Microbiol     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  353-6     Citation Subset:  IM    
Division of Life Sciences, College of Natural Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea.
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MeSH Terms
Basic-Leucine Zipper Transcription Factors
DNA-Binding Proteins / genetics,  physiology*
Fungal Proteins / genetics,  physiology*
Glutathione Transferase / biosynthesis,  genetics*
Leucine Zippers / genetics
Schizosaccharomyces / genetics*,  metabolism
Schizosaccharomyces pombe Proteins
Transcription Factors / physiology*
beta-Galactosidase / metabolism
Reg. No./Substance:
0/Basic-Leucine Zipper Transcription Factors; 0/DNA-Binding Proteins; 0/Fungal Proteins; 0/Pap1 protein, S pombe; 0/Schizosaccharomyces pombe Proteins; 0/Transcription Factors; EC Transferase; EC

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