Document Detail


Transcriptional regulation of the S-layer protein type I secretion system in Caulobacter crescentus.
MedLine Citation:
PMID:  16111836     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Gram-negative Caulobacter crescentus exports RsaA, the crystalline S-layer subunit protein using a dedicated type I secretion system. The protein and two transporter genes (rsaADE) are located together, comparable to the Escherichia coli type I hemolysin hlyCABD operon, where read through of a stem loop following hlyCA results in reduced transcription of the hlyBD. Using two genetic approaches and a direct assessment of transcription from regions 5' to the genes we learned that rsaD and rsaE were transcribed together as a separate transcript from rsaA. These results are contrary to previous assumptions about the rsaADE type I secretion gene control and add another theme to the area of type I secretion transcription regulation. It may be that to accommodate the high levels of RsaA secretion, the type I transporters must be transcribed independently from rsaA.
Authors:
Michael C Toporowski; John F Nomellini; Peter Awram; Assaf Levi; John Smit
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  251     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-09-19     Completed Date:  2006-03-10     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  29-36     Citation Subset:  IM    
Affiliation:
University of British Columbia, Department of Microbiology and Immunology, Vancouver, B.C. Canada V6T 1Z3.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / analysis,  genetics*,  metabolism*
Base Sequence
Caulobacter crescentus / genetics*,  metabolism
Gene Expression Regulation, Bacterial*
Genes, Reporter
Membrane Glycoproteins / genetics*,  metabolism*
Molecular Sequence Data
Promoter Regions, Genetic
Protein Transport / genetics*
Transcription, Genetic*
beta-Galactosidase / analysis
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Membrane Glycoproteins; 0/S-layer proteins; EC 3.2.1.23/beta-Galactosidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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