| Transcriptional profiling of immune system-related genes in postmenopausal osteoporotic versus non-osteoporotic human bone tissue. | |
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MedLine Citation:
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PMID: 19230778 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The functional interaction between the immune system and bone metabolism has been established at both molecular and cellular levels. We have used non-parametric and multidimensional expression pattern analyses to determine significantly changed mRNA profile of immune system-associated genes in postmenopausal osteoporotic (OP) vs. non-osteoporotic (NOP) bone tissue. Seven bone tissue samples from OP patients and ten bone tissue samples from NOP women were examined in our study. The transcription differences of selected 44 genes were analyzed in Taqman probe-based quantitative real-time RT-PCR system. Mann-Whitney test indicated significantly down-regulated transcription activity of 3 genes (FCGR2A, NFKB1 and SCARA3) in OP bone tissue which have prominent role in (antibody) clearance, phagocytosis, pathogen recognition and inflammatory response. According to the canonical variates analysis results, the groups of postmenopausal OP and NOP women are separable by genes coding for cytokines, co-stimulators and cell surface receptors affected in innate immunity which have high discriminatory power. Based on the complex gene expression patterns in human bone cells, we could distinguish OP and NOP states from an immunological aspect. Our data may provide further insights into the changes of the intersystem crosstalk between the immune and skeletal systems, as well as into the local immune response in the altered microenvironment of OP bone. |
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Authors:
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Bernadett Balla; János P Kósa; János Kiss; János Podani; István Takács; Aron Lazáry; Zsolt Nagy; Krisztián Bácsi; Gábor Speer; Péter Lakatos |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-02-20 |
Journal Detail:
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Title: Clinical immunology (Orlando, Fla.) Volume: 131 ISSN: 1521-7035 ISO Abbreviation: Clin. Immunol. Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-04-13 Completed Date: 2009-05-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100883537 Medline TA: Clin Immunol Country: United States |
Other Details:
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Languages: eng Pagination: 354-9 Citation Subset: IM |
Affiliation:
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1st Department of Internal Medicine, Semmelweis University, Budapest, Hungary. betti@bel1.sote.hu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aged Bone and Bones / cytology, immunology* Cells, Cultured Female Gene Expression Profiling Gene Expression Regulation* Humans Middle Aged Osteoporosis, Postmenopausal / genetics, immunology* RNA, Messenger / metabolism Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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