Document Detail


Transcriptional profile of Rous Sarcoma Virus transformed chicken embryo fibroblasts reveals new signaling targets of viral-src.
MedLine Citation:
PMID:  17448514     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Transformation of chicken fibroblasts in vitro by Rous Sarcoma Virus represents a model of cancer in which a single oncogene, viral src, uniformly and rapidly transforms primary cells in culture. We experimentally surveyed the transcriptional program affected by Rous Sarcoma Virus (RSV) in primary culture of chicken embryo fibroblasts. As a control, we used cells infected with non-transforming RSV mutant td106, in which the src gene was deleted. Using Affymetrix GeneChip Chicken Genome Arrays, we report 811 genes that were modulated more than 2.5 fold in the virus transformed cells. Among these, 409 genes were induced and 402 genes were repressed by viral src. From the repertoire of modulated genes, we selected 20 genes that were robustly changed. We then validated and quantified the transcriptional changes of most of the 20 selected genes by real-time PCR. The set of strongly induced genes contains vasoactive intestinal polypeptide, MAP kinase phosphatase 2 and follistatin, among others. The set of strongly repressed genes contains TGF beta 3, TGF beta-induced gene, and deiodinase. The function of several robustly modulated genes sheds new light on the molecular mechanism of oncogenic transformation.
Authors:
Kathryn Masker; Alicia Golden; Christian J Gaffney; Virginia Mazack; William F Schwindinger; Weizhou Zhang; Lu-Hai Wang; David J Carey; Marius Sudol
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-04-19
Journal Detail:
Title:  Virology     Volume:  364     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-06-04     Completed Date:  2007-07-18     Revised Date:  2014-05-09    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  10-20     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Cell Transformation, Viral / genetics
Cells, Cultured
Chick Embryo
DNA Primers / genetics
DNA, Viral / genetics
Gene Expression Profiling
Genes, Viral
Genes, src*
Models, Genetic
Oligonucleotide Array Sequence Analysis
Rous sarcoma virus / genetics*,  pathogenicity,  physiology
Signal Transduction
Transcription, Genetic
Grant Support
ID/Acronym/Agency:
CA29339/CA/NCI NIH HHS; DK62345/DK/NIDDK NIH HHS; P01 DK062345-02/DK/NIDDK NIH HHS; P01 DK062345-03/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA, Viral
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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