| Transcriptional and electrophysiological maturation of neocortical fast-spiking GABAergic interneurons. | |
| | |
MedLine Citation:
|
PMID: 19474331 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Fast-spiking (FS) interneurons are important elements of neocortical circuitry that constitute the primary source of synaptic inhibition in adult cortex and impart temporal organization on ongoing cortical activity. The highly specialized intrinsic membrane and firing properties that allow cortical FS interneurons to perform these functions are attributable to equally specialized gene expression, which is ultimately coordinated by cell-type-specific transcriptional regulation. Although embryonic transcriptional events govern the initial steps of cell-type specification in most cortical interneurons, including FS cells, the electrophysiological properties that distinguish adult cortical cell types emerge relatively late in postnatal development, and the transcriptional events that drive this maturational process are not known. To address this, we used mouse whole-genome microarrays and whole-cell patch clamp to characterize the transcriptional and electrophysiological maturation of cortical FS interneurons between postnatal day 7 (P7) and P40. We found that the intrinsic and synaptic physiology of FS cells undergoes profound regulation over the first 4 postnatal weeks and that these changes are correlated with primarily monotonic but bidirectional transcriptional regulation of thousands of genes belonging to multiple functional classes. Using our microarray screen as a guide, we discovered that upregulation of two-pore K(+) leak channels between P10 and P25 contributes to one of the major differences between the intrinsic membrane properties of immature and adult FS cells and found a number of other candidate genes that likely confer cell-type specificity on mature FS cells. |
| | |
Authors:
|
Benjamin W Okaty; Mark N Miller; Ken Sugino; Chris M Hempel; Sacha B Nelson |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The Journal of neuroscience : the official journal of the Society for Neuroscience Volume: 29 ISSN: 1529-2401 ISO Abbreviation: J. Neurosci. Publication Date: 2009 May |
Date Detail:
|
Created Date: 2009-05-28 Completed Date: 2009-06-23 Revised Date: 2013-03-27 |
Medline Journal Info:
|
Nlm Unique ID: 8102140 Medline TA: J Neurosci Country: United States |
Other Details:
|
Languages: eng Pagination: 7040-52 Citation Subset: IM |
Affiliation:
|
Department of Biology, Brandeis University, Waltham, Massachusetts 02453, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Action Potentials
/
drug effects,
physiology* Age Factors Analysis of Variance Animals Animals, Newborn Biophysics Electric Stimulation Excitatory Amino Acid Antagonists / pharmacology Flow Cytometry / methods Gene Expression Regulation, Developmental / drug effects, physiology Gene Regulatory Networks / drug effects, physiology* Glutamate Decarboxylase / genetics Green Fluorescent Proteins / genetics Inhibitory Postsynaptic Potentials / drug effects, genetics Interneurons / classification, drug effects, physiology* Ion Channels / genetics, metabolism Lysine / analogs & derivatives, metabolism Mice Mice, Transgenic Microarray Analysis / methods Neocortex / cytology*, growth & development* Patch-Clamp Techniques gamma-Aminobutyric Acid / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
|
R01 MH066338-07/MH/NIMH NIH HHS; R01 MH066338-09/MH/NIMH NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Excitatory Amino Acid Antagonists; 0/Ion Channels; 147336-22-9/Green Fluorescent Proteins; 56-12-2/gamma-Aminobutyric Acid; 56-87-1/Lysine; EC 4.1.1.15/Glutamate Decarboxylase; EC 4.1.1.15/glutamate decarboxylase 1; G6D6147J22/biocytin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Visual field map clusters in macaque extrastriate visual cortex.
Next Document: Cerebral Activity during the Anesthesia-Like State Induced by Mesopontine Microinjection of Pentobar...