Document Detail

Transcriptional control of polarity in Escherichia coli by cAMP.
MedLine Citation:
PMID:  6092868     Owner:  NLM     Status:  MEDLINE    
In Escherichia coli, 3'5'-adenosine cyclic monophosphate (cAMP) and its receptor protein (CAP) are known to be involved in the control of transcription initiation of catabolic operons. In previous papers we have shown that the cAMP-CAP complex is also involved as a modulator of polarity in polycistronic transcription units. Furthermore we showed that there exists a functional relationship between this complex and the transcription termination protein, Rho. In this work, we measured mRNA synthesis corresponding to the promoter proximal and distal parts of the lac and gal operons by DNA-RNA hybridization. We show that in these operons the main polarity effect is essentially transcriptional and the cAMP-CAP complex decreases polarity by interfering with premature transcription termination.
C Guidi-Rontani; A Danchin; A Ullmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular & general genetics : MGG     Volume:  195     ISSN:  0026-8925     ISO Abbreviation:  Mol. Gen. Genet.     Publication Date:  1984  
Date Detail:
Created Date:  1984-11-26     Completed Date:  1984-11-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0125036     Medline TA:  Mol Gen Genet     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  96-100     Citation Subset:  IM    
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MeSH Terms
Base Sequence
Cyclic AMP / metabolism*
DNA Restriction Enzymes
Escherichia coli / genetics*,  metabolism
Genes, Bacterial*
Lac Operon
Nucleic Acid Hybridization
Receptors, Cyclic AMP / metabolism*
Rho Factor / metabolism
Species Specificity
Transcription, Genetic*
Reg. No./Substance:
0/Receptors, Cyclic AMP; 0/Rho Factor; 60-92-4/Cyclic AMP; EC 3.1.21.-/DNA Restriction Enzymes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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