| Transcriptional control of the F(0) F(1) -ATP synthase operon of Corynebacterium glutamicum: SigmaH factor binds to its promoter and regulates its expression at different pH values. | |
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MedLine Citation:
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PMID: 23298179 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Corynebacterium glutamicum used in the amino acid fermentation industries is an alkaliphilic microorganism. Its F(0) F(1) -ATPase operon (atpBEFHAGDC) is expressed optimally at pH 9.0 forming a polycistronic (7.5 kb) and a monocistronic (1.2 kb) transcripts both starting upstream of the atpB gene. Expression of this operon is controlled by the SigmaH factor. The sigmaH gene (sigH) was cloned and shown to be co-transcribed with a small gene, cg0877, encoding a putative anti-sigma factor. A mutant deleted in the sigH gene expressed the atpBEFHAGDC operon optimally at pH 7.0 at difference of the wild-type strain (optimal expression at pH 9.0). These results suggested that the SigmaH factor is involved in pH control of expression of the F(0) F(1) ATPase operon. The SigmaH protein was expressed in Escherichia coli fused to the GST (glutathione-S-transferase) and purified to homogeneity by affinity chromatography on a GSTrap HP column. The fused protein was identified by immunodetection with anti-GST antibodies. DNA-binding studies by electrophoretic mobility shift assays showed that the SigH protein binds to a region of the atpB promoter containing the sigmaH recognition sequence (-35)TTGGAT…18nt…GTTA(-10). SigmaH plays an important role in the cascade of control of pH stress in Corynebacterium. |
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Authors:
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Mónica Barriuso-Iglesias; Carlos Barreiro; Alberto Sola-Landa; Juan F Martín |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-1-9 |
Journal Detail:
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Title: Microbial biotechnology Volume: - ISSN: 1751-7915 ISO Abbreviation: Microb Biotechnol Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-1-9 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101316335 Medline TA: Microb Biotechnol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2013 The Authors. Published by Society for Applied Microbiology and Blackwell Publishing Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
Affiliation:
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Instituto de Biotecnología de León (INBIOTEC), Parque Científico de León, Av. Real, 1, 24006, León, Spain; Departamento de Biología Molecular de la Universidad de León, Facultad de Ciencias Biológicas y Ambientales, Campus de Vegazana, s/n, 24071, León, Spain. |
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