Document Detail


Transcriptional analysis of two Rhodobacter capsulatus ferredoxins by translational fusion to Escherichia coli lacZ.
MedLine Citation:
PMID:  1959593     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Plasmids which contained the translational fusion of Escherichia coli lacZ to Rhodobacter capsulatus ferredoxin genes, fdxN and fdxA, were constructed. Effects of growth conditions on the expression of each ferredoxin were analyzed by measuring the beta-galactosidase activity in R. capsulatus which harbored a corresponding plasmid. Transcription of fdxN::lacZ, the ferredoxin I fusion gene, was regulated at least 100-fold by either NH4+ or O2 but not by illumination, confirming that fdxN belongs to the nif-gene family. Transcription of fdxA::lacZ, the ferredoxin II fusion gene, however, was constant under all the conditions surveyed, suggesting that the protein has some constitutive function(s).
Authors:
Y Suetsugu; K Saeki; H Matsubara
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEBS letters     Volume:  292     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1991 Nov 
Date Detail:
Created Date:  1992-01-07     Completed Date:  1992-01-07     Revised Date:  2006-05-01    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  13-6     Citation Subset:  IM    
Affiliation:
Department of Biology, Faculty of Science, Osaka University, Japan.
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MeSH Terms
Descriptor/Qualifier:
Escherichia coli / enzymology,  genetics*
Ferredoxins / genetics*
Genes, Bacterial
Plasmids
Protein Biosynthesis*
Restriction Mapping
Rhodobacter capsulatus / metabolism*
Transcription, Genetic*
beta-Galactosidase / metabolism
Chemical
Reg. No./Substance:
0/Ferredoxins; EC 3.2.1.23/beta-Galactosidase

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