Document Detail


Transcriptional analysis of a superoxide dismutase gene of Borrelia burgdorferi.
MedLine Citation:
PMID:  10650199     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A single superoxide dismutase (Sod) gene was identified in Borrelia burgdorferi strains, Borrelia afzelii Ple and Borrelia garinii Pbi. Recombinant enzymatic activity was detected only when sod expression was controlled by the lacZ promoter in the cloning vector. Northern blot analysis with sod- or secA-specific probes identified a common 3.7-kb transcript. Reverse transcriptase-PCR analysis confirmed that secA and sod constitute a single transcriptional unit in B. burgdorferi. A transcriptional start site of this operon, containing -10 and -35 regions of a sigma(70)-type promoter, was mapped to 100 bp upstream of the ATG start codon of secA.
Authors:
T L Nichols; C A Whitehouse; F E Austin
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  FEMS microbiology letters     Volume:  183     ISSN:  0378-1097     ISO Abbreviation:  FEMS Microbiol. Lett.     Publication Date:  2000 Feb 
Date Detail:
Created Date:  2000-03-07     Completed Date:  2000-03-07     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7705721     Medline TA:  FEMS Microbiol Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  37-42     Citation Subset:  IM    
Affiliation:
Department of Microbiology and Immunology, University of Louisville, School of Medicine, Louisville, KY, USA.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphatases / genetics,  metabolism
Animals
Bacterial Proteins*
Base Sequence
Blotting, Northern
Blotting, Southern
Borrelia burgdorferi Group / enzymology*,  genetics
Carrier Proteins / genetics,  metabolism
Electrophoresis, Polyacrylamide Gel
Escherichia coli / enzymology,  genetics
Escherichia coli Proteins*
Gene Expression Regulation, Bacterial
Humans
Ixodes / microbiology
Lyme Disease / microbiology
Membrane Transport Proteins*
Molecular Sequence Data
Promoter Regions, Genetic
Reverse Transcriptase Polymerase Chain Reaction
Superoxide Dismutase / genetics*,  metabolism
Transcription, Genetic*
Grant Support
ID/Acronym/Agency:
AI34512/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Carrier Proteins; 0/Escherichia coli Proteins; 0/Membrane Transport Proteins; 119129-39-4/SecA protein, Bacteria; EC 1.15.1.1/Superoxide Dismutase; EC 3.6.1.-/Adenosine Triphosphatases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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