| Transcriptional analysis of the Bacillus subtilis teichuronic acid operon. | |
| | |
MedLine Citation:
|
PMID: 10627039 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The cell walls of Gram-positive bacteria consist primarily of a macromolecular matrix comprising similar amounts of peptidoglycan and covalently attached anionic polymers. Under most growth conditions the anionic polymers of Bacillus subtilis are principally teichoic acids; in strain 168 these include a polyglycerol teichoic acid and a glucose/galactosamine-containing teichoic acid. However, when cultures are subjected to phosphate stress the bacterium induces a complex series of responses, one of which is the replacement of at least part of the wall teichoic acid with teichuronic acid, a non-phosphate-containing anionic polymer. In this paper the construction of a transcriptional reporter strain that facilitates the monitoring of the promoter region upstream of the tua operon involved in teichuronic acid synthesis and its controlled expression are reported. The expression of the tua operon was monitored in both phosphate-starved, non-growing batch cultures and phosphate-limited continuous cultures. We show that the transcription of the operon correlates well with the anionic polymer composition of the cell walls. |
| | |
Authors:
|
M Lahooti; C R Harwood |
Related Documents
:
|
11595219 - A rapid and sensitive procedure for determination of 5-n-acetyl neuraminic acid in lipo... 6409609 - Somatic antigens of pseudomonas aeruginosa. the structure of o-specific polysaccharide ... 18455359 - Inhibition of reverse transcriptase activity of hiv by polysaccharides of brown algae. 2046549 - Ionizing groups in lipopolysaccharides of pseudomonas cepacia in relation to antibiotic... 6190649 - Improved preparation of lipoteichoic acids. 11589969 - Structural features of a water soluble gum polysaccharide from murraya paniculata fruits. 3872009 - Differences in the haemorrhagic toxicity of aspirin between rats and mice. 10447329 - Antibacterial effects of lactoferricin b. 1919299 - The effects of temporary occlusion of the superior mesenteric vein or splenic vein on b... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Microbiology (Reading, England) Volume: 145 ( Pt 12) ISSN: 1350-0872 ISO Abbreviation: Microbiology (Reading, Engl.) Publication Date: 1999 Dec |
Date Detail:
|
Created Date: 2000-02-17 Completed Date: 2000-02-17 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 9430468 Medline TA: Microbiology Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 3409-17 Citation Subset: IM |
Affiliation:
|
Department of Microbiology and Immunology, The Medical School, Newcastle University, Newcastle upon Tyne, UK. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bacillus subtilis
/
genetics,
growth & development,
metabolism* Blotting, Northern Cell Wall / chemistry, metabolism Cloning, Molecular Gene Expression Regulation, Bacterial* Operon / genetics* Phosphates / metabolism Polymers / analysis Promoter Regions, Genetic Sequence Analysis, DNA Transcription, Genetic* Transformation, Bacterial Uronic Acids / metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Phosphates; 0/Polymers; 0/Uronic Acids; 37251-79-9/teichuronic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: S-layer protein production by Corynebacterium strains is dependent on the carbon source.
Next Document: Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS p...