Document Detail


Transcriptional analysis of the Bacillus subtilis teichuronic acid operon.
MedLine Citation:
PMID:  10627039     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cell walls of Gram-positive bacteria consist primarily of a macromolecular matrix comprising similar amounts of peptidoglycan and covalently attached anionic polymers. Under most growth conditions the anionic polymers of Bacillus subtilis are principally teichoic acids; in strain 168 these include a polyglycerol teichoic acid and a glucose/galactosamine-containing teichoic acid. However, when cultures are subjected to phosphate stress the bacterium induces a complex series of responses, one of which is the replacement of at least part of the wall teichoic acid with teichuronic acid, a non-phosphate-containing anionic polymer. In this paper the construction of a transcriptional reporter strain that facilitates the monitoring of the promoter region upstream of the tua operon involved in teichuronic acid synthesis and its controlled expression are reported. The expression of the tua operon was monitored in both phosphate-starved, non-growing batch cultures and phosphate-limited continuous cultures. We show that the transcription of the operon correlates well with the anionic polymer composition of the cell walls.
Authors:
M Lahooti; C R Harwood
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  145 ( Pt 12)     ISSN:  1350-0872     ISO Abbreviation:  Microbiology (Reading, Engl.)     Publication Date:  1999 Dec 
Date Detail:
Created Date:  2000-02-17     Completed Date:  2000-02-17     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  3409-17     Citation Subset:  IM    
Affiliation:
Department of Microbiology and Immunology, The Medical School, Newcastle University, Newcastle upon Tyne, UK.
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MeSH Terms
Descriptor/Qualifier:
Bacillus subtilis / genetics,  growth & development,  metabolism*
Blotting, Northern
Cell Wall / chemistry,  metabolism
Cloning, Molecular
Gene Expression Regulation, Bacterial*
Operon / genetics*
Phosphates / metabolism
Polymers / analysis
Promoter Regions, Genetic
Sequence Analysis, DNA
Transcription, Genetic*
Transformation, Bacterial
Uronic Acids / metabolism*
Chemical
Reg. No./Substance:
0/Phosphates; 0/Polymers; 0/Uronic Acids; 37251-79-9/teichuronic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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