Document Detail


Transcriptional analysis of the B cell germinal center reaction.
MedLine Citation:
PMID:  12604779     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The germinal center (GC) reaction is crucial for T cell-dependent immune responses and is targeted by B cell lymphomagenesis. Here we analyzed the transcriptional changes that occur in B cells during GC transit (naive B cells --> centroblasts --> centrocytes --> memory B cells) by gene expression profiling. Naive B cells, characterized by the expression of cell cycle-inhibitory and antiapoptotic genes, become centroblasts by inducing an atypical proliferation program lacking c-Myc expression, switching to a proapoptotic program, and down-regulating cytokine, chemokine, and adhesion receptors. The transition from GC to memory cells is characterized by a return to a phenotype similar to that of naive cells except for an apoptotic program primed for both death and survival and for changes in the expression of cell surface receptors including IL-2 receptor beta. These results provide insights into the dynamics of the GC reaction and represent the basis for the analysis of B cell malignancies.
Authors:
Ulf Klein; Yuhai Tu; Gustavo A Stolovitzky; Jeffrey L Keller; Joseph Haddad; Vladan Miljkovic; Giorgio Cattoretti; Andrea Califano; Riccardo Dalla-Favera
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-02-25
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  100     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-03-05     Completed Date:  2003-05-13     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2639-44     Citation Subset:  IM    
Affiliation:
Institute for Cancer Genetics and Department of Pathology, Columbia University, New York, NY 10032, USA.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis
B-Lymphocytes / cytology*,  immunology*
Cell Adhesion
Cell Division
Cell Separation
Down-Regulation
Flow Cytometry
Gene Expression Regulation*
Germinal Center*
Humans
Immunohistochemistry
Immunologic Memory
Interleukin-2 Receptor beta Subunit
Magnetics
Oligonucleotide Array Sequence Analysis
Palatine Tonsil / cytology
Phenotype
Proto-Oncogene Proteins c-myc / metabolism
Receptors, Interleukin / immunology*,  metabolism
Transcription, Genetic*
Up-Regulation
Chemical
Reg. No./Substance:
0/IL2RB protein, human; 0/Interleukin-2 Receptor beta Subunit; 0/Proto-Oncogene Proteins c-myc; 0/Receptors, Interleukin
Comments/Corrections

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