| Transcriptional analysis of the B cell germinal center reaction. | |
| | |
MedLine Citation:
|
PMID: 12604779 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The germinal center (GC) reaction is crucial for T cell-dependent immune responses and is targeted by B cell lymphomagenesis. Here we analyzed the transcriptional changes that occur in B cells during GC transit (naive B cells --> centroblasts --> centrocytes --> memory B cells) by gene expression profiling. Naive B cells, characterized by the expression of cell cycle-inhibitory and antiapoptotic genes, become centroblasts by inducing an atypical proliferation program lacking c-Myc expression, switching to a proapoptotic program, and down-regulating cytokine, chemokine, and adhesion receptors. The transition from GC to memory cells is characterized by a return to a phenotype similar to that of naive cells except for an apoptotic program primed for both death and survival and for changes in the expression of cell surface receptors including IL-2 receptor beta. These results provide insights into the dynamics of the GC reaction and represent the basis for the analysis of B cell malignancies. |
| | |
Authors:
|
Ulf Klein; Yuhai Tu; Gustavo A Stolovitzky; Jeffrey L Keller; Joseph Haddad; Vladan Miljkovic; Giorgio Cattoretti; Andrea Califano; Riccardo Dalla-Favera |
Related Documents
:
|
18631289 - Microgenomic analysis reveals cell type-specific gene expression patterns between ray a... 20705589 - Inhibition of epithelial to mesenchymal transition in metastatic breast carcinoma cells... 14980499 - Effects of pu.1-induced mouse calcium-calmodulin-dependent kinase i-like kinase (cklik)... 20184659 - Global transcriptomic analysis of murine embryonic stem cell-derived brachyury (t) cells. 16357179 - Motexafin gadolinium and zinc induce oxidative stress responses and apoptosis in b-cell... 20957409 - Influence of zinc and zinc chelator on ht-29 colorectal cell line. 3594489 - Expression of hla-dr antigens on tumour cells does not contribute to skin reactivity to... 23073799 - Isolation and identification of cancer stem-like cells from side population of human pr... 23574319 - Ro52- and ro60-specific b cell pattern in the salivary glands of patients with primary ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2003-02-25 |
Journal Detail:
|
Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 100 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2003 Mar |
Date Detail:
|
Created Date: 2003-03-05 Completed Date: 2003-05-13 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
|
Languages: eng Pagination: 2639-44 Citation Subset: IM |
Affiliation:
|
Institute for Cancer Genetics and Department of Pathology, Columbia University, New York, NY 10032, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Apoptosis B-Lymphocytes / cytology*, immunology* Cell Adhesion Cell Division Cell Separation Down-Regulation Flow Cytometry Gene Expression Regulation* Germinal Center* Humans Immunohistochemistry Immunologic Memory Interleukin-2 Receptor beta Subunit Magnetics Oligonucleotide Array Sequence Analysis Palatine Tonsil / cytology Phenotype Proto-Oncogene Proteins c-myc / metabolism Receptors, Interleukin / immunology*, metabolism Transcription, Genetic* Up-Regulation |
| Chemical | |
Reg. No./Substance:
|
0/IL2RB protein, human; 0/Interleukin-2 Receptor beta Subunit; 0/Proto-Oncogene Proteins c-myc; 0/Receptors, Interleukin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Cdc42-interacting protein 4 binds to huntingtin: neuropathologic and biological evidence for a role ...
Next Document: The FKBP-associated protein FAP48 is an antiproliferative molecule and a player in T cell activation...