Document Detail

Transcriptional activation of apurinic/apyrimidinic endonuclease (Ape, Ref-1) by oxidative stress requires CREB.
MedLine Citation:
PMID:  10441516     Owner:  NLM     Status:  MEDLINE    
Apurinic/apyrimidinic endonuclease (APE alias Ref-1) is a multifunctional enzyme involved in DNA repair and redox regulation of transcription factors (e.g., AP-1). It also acts as a repressor of its own and other genes. Recently, it was shown that the level of APE mRNA and protein is enhanced upon treatment of cells with oxidative agents, such as hydrogen peroxide (H(2)O(2)), which gives rise to an adaptive response of cells to oxidative stress. Induction of APE is due to APE promoter activation. To elucidate the mechanism of transcriptional activation of APE by oxidative agents, we introduced mutations into the cloned human APE promoter and checked its activity in transient transfection assays. Here we demonstrate that mutational inactivation of a CREB binding site (CRE) present within the promoter completely abolished APE promoter activation by H(2)O(2), indicating that CREB is required for APE induction. The CRE element in the context of the APE promoter sequence binds c-Jun and ATF-2, which was shown in gel retardation experiments. Under conditions of induction of APE by H(2)O(2), the expression of c-Jun was significantly enhanced, which supports the view that induction of c-Jun is involved in signaling leading to APE promoter activation by oxidative stress.
S Grösch; B Kaina
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  261     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1999 Aug 
Date Detail:
Created Date:  1999-09-09     Completed Date:  1999-09-09     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  859-63     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Academic Press.
Institute of Toxicology, University of Mainz, Obere Zahlbacher Strasse 67, Mainz, D-55131, Germany.
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MeSH Terms
Activating Transcription Factor 2
Binding Sites
CHO Cells
Carbon-Oxygen Lyases / genetics*
Cyclic AMP Response Element-Binding Protein / metabolism,  physiology*
DNA-(Apurinic or Apyrimidinic Site) Lyase*
Hydrogen Peroxide / pharmacology
Mutagenesis, Site-Directed
Oxidants / pharmacology
Oxidative Stress*
Polymerase Chain Reaction
Promoter Regions, Genetic
Proto-Oncogene Proteins c-jun / metabolism
Transcription Factors / metabolism
Transcription, Genetic*
Reg. No./Substance:
0/ATF2 protein, human; 0/Activating Transcription Factor 2; 0/Cyclic AMP Response Element-Binding Protein; 0/Oxidants; 0/Proto-Oncogene Proteins c-jun; 0/Transcription Factors; 7722-84-1/Hydrogen Peroxide; EC 4.2.-/Carbon-Oxygen Lyases; EC protein, human; EC or Apyrimidinic Site) Lyase

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