| Transcriptional activation by bidirectional RNA polymerase II elongation over a silent promoter. | |
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MedLine Citation:
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PMID: 16113646 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Transcriptional interference denotes negative cis effects between promoters. Here, we show that promoters can also interact positively. Bidirectional RNA polymerase II (Pol II) elongation over the silent human endogenous retrovirus (HERV)-K 18 promoter (representative of 2.5 x 10(3) similar promoters genomewide) activates transcription. In tandem constructs, an upstream promoter activates HERV-K 18 transcription. This is abolished by inversion of the upstream promoter, or by insertion of a poly(A) signal between the promoters; transcription is restored by poly(A) signal mutants. TATA-box mutants in the upstream promoter reduce HERV-K 18 transcription. Experiments with the same promoters in a convergent orientation produce similar effects. A small promoter deletion partially restores HERV-K 18 activity, consistent with activation resulting from repressor repulsion by the elongating Pol II. Transcriptional elongation over this class of intragenic promoters will generate co-regulated sense-antisense transcripts, or, alternatively initiating transcripts, thus expanding the diversity and complexity of the human transcriptome. |
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Authors:
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Olivier Leupin; Catia Attanasio; Samuel Marguerat; Myriam Tapernoux; Stylianos E Antonarakis; Bernard Conrad |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: EMBO reports Volume: 6 ISSN: 1469-221X ISO Abbreviation: EMBO Rep. Publication Date: 2005 Oct |
Date Detail:
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Created Date: 2005-10-13 Completed Date: 2006-01-31 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 100963049 Medline TA: EMBO Rep Country: England |
Other Details:
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Languages: eng Pagination: 956-60 Citation Subset: IM |
Affiliation:
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Department of Genetic Medicine & Development, University of Geneva Medical School, CMU, 1211 Geneva, Switzerland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Enhancer Elements, Genetic Humans Mice Mutation Plasmids / genetics RNA Polymerase II / genetics* Retroelements / genetics TATA Box / genetics, physiology* Transcription, Genetic Transcriptional Activation* |
| Chemical | |
Reg. No./Substance:
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0/Retroelements; EC 2.7.7.-/RNA Polymerase II |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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