| Transcriptional activation of JC virus early promoter by phorbol ester and interleukin-1beta: critical role of nuclear factor-1. | |
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MedLine Citation:
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PMID: 15327898 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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JC virus causes the fatal demyelinating disease, progressive multifocal leukoencephalopathy (PML) under immunosuppressive states such as AIDS. During the pathogenesis of AIDS, HIV-infected microglia secrete cytokines including interleukin-1 and tumor necrosis factor-alpha (TNF-alpha), which affect neuronal cells resulting in dysfunction of the CNS. We hypothesized that extracellular stimuli released from HIV-infected microglia may reactivate JC virus by affecting neighboring oligodendrocytes. In the present study, we found that phorbol myristate acetate (PMA) and interleukin-1beta (IL-1beta) dramatically increased JC virus transcription in glial cells. Site-directed mutagenesis and gel shift analyses revealed that PMA and IL-1beta strongly induced nuclear factor-1 (NF-1) binding to the JC virus enhancer region, increasing transcriptional activity of the viral early promoter. Additionally, we demonstrated that protein kinase C (PKC) pathways were involved in the PMA/IL-1beta-mediated up-regulation of the JC virus early promoter. These findings may represent one of the possible mechanisms for higher incidence of PML among AIDS patients. |
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Authors:
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So-Young Kim; Eung-Chil Choi; Yeong Woo Jo; John W Henson; Hee-Sun Kim |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Virology Volume: 327 ISSN: 0042-6822 ISO Abbreviation: Virology Publication Date: 2004 Sep |
Date Detail:
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Created Date: 2004-08-25 Completed Date: 2004-09-28 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0110674 Medline TA: Virology Country: United States |
Other Details:
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Languages: eng Pagination: 60-9 Citation Subset: IM |
Affiliation:
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Department of Neuroscience, Ewha Institute of Neuroscience and Medical Research Center, Ewha Womans University School of Medicine, Seoul, South Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Binding Sites CCAAT-Enhancer-Binding Proteins / chemistry, metabolism* Cell Line, Tumor Humans Interleukin-1 / pharmacology* JC Virus / genetics, metabolism Molecular Sequence Data Mutagenesis, Site-Directed NFI Transcription Factors Phorbol Esters / pharmacology* Promoter Regions, Genetic / genetics, physiology* Transcription Factors / chemistry, metabolism* Transcriptional Activation / drug effects* |
| Chemical | |
Reg. No./Substance:
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0/CCAAT-Enhancer-Binding Proteins; 0/Interleukin-1; 0/NFI Transcription Factors; 0/Phorbol Esters; 0/Transcription Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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