Document Detail


Transcriptional mediators Kto and Skd are involved in the regulation of the IMD pathway and anti-Plasmodium defense in Anopheles gambiae.
MedLine Citation:
PMID:  23049816     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The malarial parasite Plasmodium must complete a complex lifecycle in its Anopheles mosquito host, the main vector for Plasmodium. The mosquito resists infection with the human malarial parasite P. falciparum by engaging the NF-κB immune signaling pathway, IMD. Here we show that the conserved transcriptional mediators Kto and Skd are involved in the regulation of the mosquito IMD pathway. RNAi-mediated depletion of Kto and Skd in the Anopheles gambiae cell line L5-3 resulted in a decrease in the transcript abundance of Cec1, which is controlled by the IMD pathway. Silencing the two genes also resulted in an increased susceptibility of the mosquito to bacterial and Plasmodium falciparum infection, but not to infection with the rodent malaria parasite P. berghei. We also showed that Kto and Skd are not transcriptional co-activators of Rel2 or other key factors of the IMD pathway; however, they participate in the regulation of the IMD pathway, which is crucial for the mosquito's defense against P. falciparum.
Authors:
Yang Chen; Yuemei Dong; Simone Sandiford; George Dimopoulos
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Publication Detail:
Type:  Journal Article     Date:  2012-09-25
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-10-10     Completed Date:  2013-04-22     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e45580     Citation Subset:  IM    
Affiliation:
W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anopheles gambiae / genetics*,  immunology*,  parasitology
Cell Line
Female
Gene Expression Regulation
Gene Silencing
Host-Parasite Interactions
Humans
Insect Proteins / antagonists & inhibitors,  genetics*,  metabolism
Plasmodium berghei / physiology*
Plasmodium falciparum / physiology*
RNA, Small Interfering / genetics
Signal Transduction
Species Specificity
Transcription Factors / antagonists & inhibitors,  genetics*,  metabolism
Transcription, Genetic
Chemical
Reg. No./Substance:
0/Insect Proteins; 0/RNA, Small Interfering; 0/Transcription Factors
Comments/Corrections

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