Document Detail


Transcriptional differences between rhesus embryonic stem cells generated from in vitro and in vivo derived embryos.
MedLine Citation:
PMID:  23028448     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Numerous studies have focused on the transcriptional signatures that underlie the maintenance of embryonic stem cell (ESC) pluripotency. However, it remains unclear whether ESC retain transcriptional aberrations seen in in vitro cultured embryos. Here we report the first global transcriptional profile comparison between ESC generated from either in vitro cultured or in vivo derived primate embryos by microarray analysis. Genes involved in pluripotency, oxygen regulation and the cell cycle were downregulated in rhesus ESC generated from in vitro cultured embryos (in vitro ESC). Significantly, several gene differences are similarly downregulated in preimplantation embryos cultured in vitro, which have been associated with long term developmental consequences and disease predisposition. This data indicates that prior to derivation, embryo quality may influence the molecular signature of ESC lines, and may differentially impact the physiology of cells prior to or following differentiation.
Authors:
Alexandra J Harvey; Shihong Mao; Claudia Lalancette; Stephen A Krawetz; Carol A Brenner
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-09-18
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-10-02     Completed Date:  2013-03-19     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e43239     Citation Subset:  IM    
Affiliation:
Department of Physiology, Wayne State University, Detroit, Michigan, United States of America. ajharvey@unimelb.edu.au
Data Bank Information
Bank Name/Acc. No.:
GEO/GSE25198
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MeSH Terms
Descriptor/Qualifier:
Animals
Blastocyst / metabolism
Cluster Analysis
Embryo, Mammalian / embryology,  metabolism
Embryonic Stem Cells / metabolism*
Gene Expression Profiling*
Gene Expression Regulation, Developmental*
Gene Regulatory Networks
Macaca mulatta
Oxygen Consumption / genetics
Reproducibility of Results
Signal Transduction
Transcription Factors / genetics
Transcription, Genetic*
Grant Support
ID/Acronym/Agency:
HD045966/HD/NICHD NIH HHS; HD046553/HD/NICHD NIH HHS; RR015395/RR/NCRR NIH HHS; RR021881/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Transcription Factors
Comments/Corrections

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