| Transcriptional Differences between Rhesus Embryonic Stem Cells Generated from In Vitro and In Vivo Derived Embryos. | |
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MedLine Citation:
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PMID: 23028448 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Numerous studies have focused on the transcriptional signatures that underlie the maintenance of embryonic stem cell (ESC) pluripotency. However, it remains unclear whether ESC retain transcriptional aberrations seen in in vitro cultured embryos. Here we report the first global transcriptional profile comparison between ESC generated from either in vitro cultured or in vivo derived primate embryos by microarray analysis. Genes involved in pluripotency, oxygen regulation and the cell cycle were downregulated in rhesus ESC generated from in vitro cultured embryos (in vitro ESC). Significantly, several gene differences are similarly downregulated in preimplantation embryos cultured in vitro, which have been associated with long term developmental consequences and disease predisposition. This data indicates that prior to derivation, embryo quality may influence the molecular signature of ESC lines, and may differentially impact the physiology of cells prior to or following differentiation. |
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Authors:
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Alexandra J Harvey; Shihong Mao; Claudia Lalancette; Stephen A Krawetz; Carol A Brenner |
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Publication Detail:
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Type: Journal Article Date: 2012-09-18 |
Journal Detail:
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Title: PloS one Volume: 7 ISSN: 1932-6203 ISO Abbreviation: PLoS ONE Publication Date: 2012 |
Date Detail:
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Created Date: 2012-10-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101285081 Medline TA: PLoS One Country: United States |
Other Details:
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Languages: eng Pagination: e43239 Citation Subset: IM |
Affiliation:
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Department of Physiology, Wayne State University, Detroit, Michigan, United States of America. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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