Document Detail


Transcription within condensed chromatin: Steric hindrance facilitates elongation.
MedLine Citation:
PMID:  20197036     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
During eukaryotic transcription, RNA-polymerase activity generates torsional stress in DNA, having a negative impact on the elongation process. Using our previous studies of chromatin fiber structure and conformational transitions, we suggest that this torsional stress can be alleviated, thanks to a tradeoff between the fiber twist and nucleosome conformational transitions into an activated state named "reversome". Our model enlightens the origin of polymerase pauses, and leads to the counterintuitive conclusion that chromatin-organized compaction might facilitate polymerase progression. Indeed, in a compact and well-structured chromatin loop, steric hindrance between nucleosomes enforces sequential transitions, thus ensuring that the polymerase always meets a permissive nucleosomal state.
Authors:
Christophe Bécavin; Maria Barbi; Jean-Marc Victor; Annick Lesne
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biophysical journal     Volume:  98     ISSN:  1542-0086     ISO Abbreviation:  Biophys. J.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-03-03     Completed Date:  2010-05-19     Revised Date:  2013-05-30    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  824-33     Citation Subset:  IM    
Copyright Information:
2010 Biophysical Society. Published by Elsevier Inc. All rights reserved.
Affiliation:
Institut des Hautes Etudes Scientifiques, Bures-sur-Yvette, France.
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MeSH Terms
Descriptor/Qualifier:
Biophysical Phenomena*
Chromatin / genetics*
DNA-Directed RNA Polymerases / metabolism
Kinetics
Models, Biological
Nucleic Acid Conformation
Nucleosomes / chemistry,  metabolism
Thermodynamics
Time Factors
Transcription, Genetic*
Chemical
Reg. No./Substance:
0/Chromatin; 0/Nucleosomes; EC 2.7.7.6/DNA-Directed RNA Polymerases
Comments/Corrections

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