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Transcription of the viral genome in cell lines transformed by simian virus 40. I. Mapping of virus-specific nuclear RNAs.
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MedLine Citation:
PMID:  6243776     Owner:  NLM     Status:  MEDLINE    
Mapping of virus-specific nuclear transcripts was carried out in three lines of rat cells transformed by SV40. Each of these cell lines contained a single copy of integrated viral DNA with identified regions adjacent to cell DNA (1). The main virus-specific nuclear transcript in all of these cell lines was shown to be complementary to the minus strand of the early region in SV40 genome. Each cell nucleus contained approximately 50 copies of these RNAs. Transcripts complementary to both strands of the late region in viral genome were also detectable in all of these cell lines. Its content varied depending on the cell line and was 20-50-fold less than that of the main virus-specific transcript. All the regions of integrated SV40 genome in isolated nuclei of transformed cells were equally sensitive to pancreatic DNase I treatment suggesting that the whole viral genome served as a template for RNA synthesis in these cell lines.
P M Chumakov
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nucleic acids research     Volume:  8     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1980 Jan 
Date Detail:
Created Date:  1980-04-17     Completed Date:  1980-04-17     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  111-25     Citation Subset:  IM    
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MeSH Terms
Cell Line
Cell Nucleus / metabolism*
Cell Transformation, Viral*
DNA Restriction Enzymes
Genes, Viral*
Nucleic Acid Hybridization
Nucleic Acid Renaturation
RNA, Viral / biosynthesis*
Simian virus 40 / metabolism*
Transcription, Genetic*
Reg. No./Substance:
0/RNA, Viral; EC 3.1.21.-/DNA Restriction Enzymes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
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Print publication date: Day: 11 Month: 1 Year: 1980
Volume: 8 Issue: 1
First Page: 111 Last Page: 125
ID: 327246
PubMed Id: 6243776

Transcription of the viral genome in cell lines transformed by simian virus 40. I. Mapping of virus-specific nuclear RNAs.
P M Chumakov

Article Categories:
  • Research Article

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