| Transcription inhibition using oligonucleotide-modified gold nanoparticles. | |
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MedLine Citation:
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PMID: 16984126 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The capture of T7 RNA polymerase using double-stranded promoter DNA on the surface of gold nanoparticles has been demonstrated. The competitive binding and inhibition of T7 RNA polymerase due to specific interactions on the nanoparticle surface represents a transcription factor decoy approach in a model system. The efficiency of inhibition was determined for various nanoparticle sizes, surface coverage, and linker length for double-stranded promoter DNA on gold nanoparticles. The experiments provide a basis for determining the accessibility of binding sites on nanoparticle surfaces for applications involving cell targeting or the use of nanoparticles as binding agents in solution. |
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Authors:
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Chiamaka Agbasi-Porter; Jessica Ryman-Rasmussen; Stefan Franzen; Daniel Feldheim |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Bioconjugate chemistry Volume: 17 ISSN: 1043-1802 ISO Abbreviation: Bioconjug. Chem. Publication Date: 2006 Sep-Oct |
Date Detail:
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Created Date: 2006-09-20 Completed Date: 2007-05-15 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9010319 Medline TA: Bioconjug Chem Country: United States |
Other Details:
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Languages: eng Pagination: 1178-83 Citation Subset: IM |
Affiliation:
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Department of Chemistry, North Carolina State University, Raleigh, 27695, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line DNA-Directed RNA Polymerases / genetics, metabolism Gold / chemistry*, metabolism Humans Metal Nanoparticles / chemistry* Oligonucleotides / chemistry*, metabolism Promoter Regions, Genetic Transcription, Genetic* Viral Proteins / genetics, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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CA098194/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Oligonucleotides; 0/Viral Proteins; 7440-57-5/Gold; EC 2.7.7.-/bacteriophage T7 RNA polymerase; EC 2.7.7.6/DNA-Directed RNA Polymerases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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