| Transcription of human endogenous retroviral sequences related to mouse mammary tumor virus in human breast and placenta: similar pattern in most malignant and nonmalignant breast tissues. | |
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MedLine Citation:
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PMID: 9100993 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The human genome contains a large variety of sequences related to the mouse mammary tumor virus (MMTV). We have investigated the range of expression of human endogenous retroviral sequences (HERVs) related to MMTV (human MMTV-like; HML) as RNA in 60 breast cancers, 8 nonmalignant breast tissues, and 9 placentas. This was monitored using HML group-specific oligonucleotide probes in hybridizations toward PCR amplificates of HML pol sequences and internal control. The degree of expression of five HML groups varied between individuals and between tissues. On average, all HML groups were less expressed in breast tissues than in placenta. The hybridization signals of some HML RNAs were strongly correlated, indicating a nonstochastic mechanism and a concerted regulation of their expression. The PCR product from one breast cancer (BC 6), which gave an exceptionally high expression with probe hml-6, with a 20 times stronger signal than the rest of the cancers, was cloned and sequenced. The HML-6 transcript sequences were homogeneous in BC 6. The most predominant clone derived from the cancer was used as a probe in Southern hybridizations. The same restriction fragments were detected in human breast tissues, PBMCs (peripheral blood mononuclear cells), and breast cancer cell lines, except for one of the breast cancers and one of the nonmalignant breast tissues, which gave different banding patterns. A comparison of HML expression in normal and malignant breast tissue from the same individual would have been more precise than our comparison of samples from different persons. Bearing this limitation in mind, with a single exception, human MMTV-like sequences were not more actively expressed in malignant than in nonmalignant breast tissues. Nevertheless, an interesting diversity in their expression, especially between individuals, was found. |
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Authors:
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H Yin; P Medstrand; M L Andersson; A Borg; H Olsson; J Blomberg |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: AIDS research and human retroviruses Volume: 13 ISSN: 0889-2229 ISO Abbreviation: AIDS Res. Hum. Retroviruses Publication Date: 1997 Apr |
Date Detail:
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Created Date: 1997-06-24 Completed Date: 1997-06-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8709376 Medline TA: AIDS Res Hum Retroviruses Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 507-16 Citation Subset: IM; X |
Affiliation:
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Department of Infectious Diseases and Clinical Microbiology, University of Uppsala, Sweden. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/U62098 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Aged, 80 and over Amino Acid Sequence Animals Base Sequence Breast / pathology, virology* Breast Neoplasms / pathology, virology* Cell Line DNA, Complementary DNA, Viral Female Gene Expression Genome, Viral Humans Mammary Tumor Virus, Mouse / genetics* Mice Middle Aged Molecular Sequence Data Placenta / pathology, virology* Retroviridae / genetics* Sequence Homology, Nucleic Acid Transcription, Genetic* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 0/DNA, Viral |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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