| Transcription of the genome of the filamentous bacteriophage cf from both plus and minus DNA strands. | |
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MedLine Citation:
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PMID: 10191188 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The filamentous bacteriophage cf infects the bacterium Xanthomonas campestris pv. citri. Northern blot analysis with probes derived from various restriction fragments of cf replicative form (RF) DNA has revealed the presence of five major phage-specific transcripts in infected cells. Four of these transcripts were shown to be derived from the region of the cf genome extending from gene II to gene VIII and are consistent with the cascade model of transcription proposed for Ff coliphages. These transcripts overlap with each other and terminate upstream of an efficient Rho-independent transcription terminator. Unlike the well-characterized Ff phages, in which only the minus strand of viral DNA serves as a transcription template, both strands of the RF DNA of phage cf appeared to be transcribed. Thus one of the five major cf transcripts was shown to be derived from a region of the viral minus strand that contains an open reading frame encoding a putative polypeptide of 165 amino acids. Primer extension analysis mapped the transcriptional initiation site of this RNA to a cytosine residue at position 870. A partial transcription map of phage cf revealed two independent regions of transcriptional activity. The region with the highest activity coincides with that encoding the polypeptides required in the largest amounts during the cf infection cycle. |
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Authors:
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H J Wang; C M Cheng; C N Wang; T T Kuo |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Virology Volume: 256 ISSN: 0042-6822 ISO Abbreviation: Virology Publication Date: 1999 Apr |
Date Detail:
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Created Date: 1999-06-01 Completed Date: 1999-06-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0110674 Medline TA: Virology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 228-32 Citation Subset: IM |
Copyright Information:
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Copyright 1999 Academic Press. |
Affiliation:
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Institute of Molecular Biology and Institute of Botany Academia Sinica, Nankang, Taipei, 115, Republic of China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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5' Untranslated Regions Bacteriophages / genetics* DNA, Viral* Genome, Viral RNA, Viral Transcription, Genetic* Xanthomonas campestris / virology* |
| Chemical | |
Reg. No./Substance:
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0/5' Untranslated Regions; 0/DNA, Viral; 0/RNA, Viral |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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