Document Detail


Transcription of gD and gI genes in BHV1-infected cells.
MedLine Citation:
PMID:  23151787     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Glycoprotein D (gD) and glycoprotein I (gI) genes of bovine herpes virus 1 (BHV1) are contiguous genes with 141 bp region between the two open reading frames (ORFs). Expression of gD and gI from a bicistronic construct containing complete gD and gI gene has been reported either through internal ribosome entry site (IRES)-like element or through the scanning and leakage model (Mukhopadhyay 2000). We here show by computational and experimental means that gD is expressed solely as bicistronic transcript comprising gD and gI coding region in BHV1-infected cells. gI ORF was also shown to express separately. An IRES-like element was also predicted by IRES predicting software in the middle of the gD coding region; within that region a putative promoter was also identified by promoterscan. The intergenic region between the two ORF showed extensive secondary structure which brings the stop codon of gD very close to start codon of gI gene. gD gene transcript in BHV1-infected cells was solely bicistronic. gI transcript was also present in the BHV1-infected cells but in low copy number. The results indicate that gI is probably transcribed from its own transcript in BHV1-infected cells.
Authors:
Sumit Chowdhury; Bhaskar Sharma
Related Documents :
24475317 - A pre- and co-knockdown of rnaset enzyme, eri-1, enhances the efficiency of rnai induce...
23661447 - Molecular cloning and tissue expression analyses of two novel pepper genes: heterotrime...
23508637 - Genomic copy number alterations with transcriptional deregulation at 6p identify an agg...
23070227 - Maternal mga is required for wnt signaling and organizer formation in the early xenopus...
20215587 - The arabidopsis thaliana myo-inositol 1-phosphate synthase1 gene is required for myo-in...
11004177 - The toryz (yeck bisz) operon encodes a third respiratory trimethylamine n-oxide reducta...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biosciences     Volume:  37     ISSN:  0973-7138     ISO Abbreviation:  J. Biosci.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-15     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100809     Medline TA:  J Biosci     Country:  India    
Other Details:
Languages:  eng     Pagination:  971-7     Citation Subset:  IM    
Affiliation:
Biochemistry Division, IVRI, Izatnagar 243 122, India.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Efficient multi-site-directed mutagenesis directly from genomic template.
Next Document:  Cx43, ZO-1, alpha-catenin and beta-catenin in cataractous lens epithelial cells.