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Transcription factors interacting with herpes simplex virus alpha gene promoters in sensory neurons.
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MedLine Citation:
PMID:  8559654     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Interference with VP16-mediated activation of herpes virus immediate-early (or alpha) genes is thought to be the major cause of establishing viral latency in sensory neurons. This could be brought about by lack of a key activating transcription factor(s) or active repression. In this study we find that sensory neurons express all important components for VP16-mediated alpha gene induction, such as the POU transcription factor Oct-1, host cell factor (HCF) and GABP alpha/beta. However, Oct-1 and GABP alpha/beta are only present at low levels and the VP16-induced complex (VIC) appears different. We do not find protein expression of the transcription factor Oct-2, implicated by others as an alpha gene repressor. The POU factor N-Oct3 (Brn 2 or POU3F2) is also present in sensory neurons and binds viral TAATGARAT motifs with higher affinity than Oct-1, indicating that it may be a candidate repressor for competitive binding to TAATGARAT motifs. When transfected into HeLa cells, where Oct-1 and GABP alpha/beta are highly abundant, N-Oct3 represses model promoters with multimerized TAATGARAT motifs, but fails to repress complete alpha gene promoters. Taken together our findings suggest that modulation of alpha gene promoters could contribute to viral latency when low concentrations of the activating transcription factors Oct-1 and GABP alpha/beta prevail. Our data, however, refute the notion that competing Oct factors are able to block alpha gene transcription to achieve viral latency.
Authors:
M Hagmann; O Georgiev; W Schaffner; P Douville
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nucleic acids research     Volume:  23     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1995 Dec 
Date Detail:
Created Date:  1996-02-26     Completed Date:  1996-02-26     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  4978-85     Citation Subset:  IM    
Affiliation:
Institut für Molekularbiologie II der Universität Zürich, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Hela Cells
Herpes Simplex Virus Protein Vmw65 / metabolism*
Humans
Immediate-Early Proteins / genetics*
Mice
Molecular Sequence Data
Neurons, Afferent / metabolism*
Promoter Regions, Genetic / genetics*
Simplexvirus / genetics,  metabolism*
Transcription Factors / genetics,  metabolism*
Transcriptional Activation*
Transfection
Ubiquitin-Protein Ligases
Chemical
Reg. No./Substance:
0/Herpes Simplex Virus Protein Vmw65; 0/Immediate-Early Proteins; 0/Transcription Factors; 0/herpes simplex virus, type 1 protein ICP4; EC 6.3.2.19/Ubiquitin-Protein Ligases; EC 6.3.2.19/Vmw110 protein, Human herpesvirus 1
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Full Text
Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
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Print publication date: Day: 25 Month: 12 Year: 1995
Volume: 23 Issue: 24
First Page: 4978 Last Page: 4985
ID: 307502
PubMed Id: 8559654
Publisher Item Identifier: 5w0177

Transcription factors interacting with herpes simplex virus alpha gene promoters in sensory neurons.
M Hagmann
O Georgiev
W Schaffner
P Douville
Institut f?r Molekularbiologie II der Universit?t Z?rich, Switzerland.



Article Categories:
  • Research Article


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