| Transcription factors Stat3 and Stat5b are present in rat liver nuclei late in an acute phase response and bind interleukin-6 response elements. | |
| | |
MedLine Citation:
|
PMID: 8530402 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Proteins binding at the interleukin-6 response element of the rat alpha 2 macroglobulin gene were purified by a combination of chromatographic procedures including binding site-specific DNA-affinity chromatography as the principal step. Three polypeptides of 92, 91, and 86 kDa were enriched approximately 6,300-fold from nuclei of rat livers excised 12 h after the induction of an experimental acute phase response. Amino acid sequence analysis identified the 86- and 91-kDa species as two forms of the transcription factor Stat3 and the 92-kDa species as the factor Stat5b. This identification was confirmed by gel mobility shift-supershift experiments using specific antisera for Stat3 and Stat5. Unexpectedly, activated Stat5 was also detected in the nuclei of untreated control rats. cDNA clones representing Stat3 and two isoforms of Stat5b were isolated from a cDNA library prepared with mRNA from rat livers excised at the peak of an experimental acute phase response. Full-length Stat5b, predicted from cDNA, consisted of 786 amino acids, while the variant Stat5b delta 40C lacked 41 amino acids at the COOH terminus. The amino acid sequence of rat Stat5b showed 26.7% overall identity with rat Stat3, 87.3% with sheep Stat5a, 92.5% with murine Stat5a, and 98.7% with murine Stat5b. |
| | |
Authors:
|
J A Ripperger; S Fritz; K Richter; G M Hocke; F Lottspeich; G H Fey |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The Journal of biological chemistry Volume: 270 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1995 Dec |
Date Detail:
|
Created Date: 1996-01-26 Completed Date: 1996-01-26 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 29998-30006 Citation Subset: IM |
Affiliation:
|
Chair of Genetics, University of Erlangen-Nürnberg, Federal Republic of Germany. |
| Data Bank Information | |
Bank Name/Acc. No.:
|
GENBANK/X91810; X91988 |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acute-Phase Proteins
/
biosynthesis*,
chemistry,
isolation & purification Amino Acid Sequence Animals Base Sequence Binding Sites Cell Nucleus / metabolism* Chromatography, Affinity Chromatography, Gel Chromatography, Ion Exchange Cloning, Molecular Consensus Sequence Conserved Sequence DNA / metabolism DNA Primers DNA-Binding Proteins / biosynthesis*, chemistry, isolation & purification Interleukin-6 / pharmacology* Liver / metabolism* Male Mice Milk Proteins* Molecular Sequence Data Molecular Weight Mutagenesis, Site-Directed Polymerase Chain Reaction Rats Rats, Sprague-Dawley STAT3 Transcription Factor STAT5 Transcription Factor Sequence Homology, Amino Acid Sheep Trans-Activators / biosynthesis*, chemistry, isolation & purification alpha-Macroglobulins / biosynthesis*, genetics* |
| Chemical | |
Reg. No./Substance:
|
0/Acute-Phase Proteins; 0/DNA Primers; 0/DNA-Binding Proteins; 0/Interleukin-6; 0/Milk Proteins; 0/STAT3 Transcription Factor; 0/STAT5 Transcription Factor; 0/Stat3 protein, mouse; 0/Stat3 protein, rat; 0/Stat5a protein, mouse; 0/Stat5a protein, rat; 0/Stat5b protein, mouse; 0/Stat5b protein, rat; 0/Trans-Activators; 0/alpha-Macroglobulins; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A physiological role for Saccharomyces cerevisiae copper/zinc superoxide dismutase in copper bufferi...
Next Document: Role of the catalytic serine in the interactions of serine proteinases with protein inhibitors of th...