Document Detail


Transcription factors NF-kappaB and AP-1/c-fos in cell response to nocodazole.
MedLine Citation:
PMID:  11817569     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of nocodazole (an agent causing degradation of microtubules in vitro) on the content of transcription factors NF-kappaB and AP-1/c-fos in the nuclei of cultured cells CHO-K1 and HeLa, as well as on the activity of these factors in the nuclei of T-cell hybridoma 2B4 were studied. Immunoblotting, immunofluorescence, and gel retardation techniques revealed that in all cell lines studied nocodazole induced rapid and considerable activation (an increased content in the nuclei) of transcription factor AP-1/c-fos. In 2B4 cells, nocodazole had no effect on the activity of NF-kappaB, but in CHO-K1 and HeLa cells it caused a transient decrease, sometimes followed by an increase in the content of subunit p50. Besides, nocodazole inhibited proteolytic degradation of NF-kappaB in CHO-K1 and HeLa cells and caused a short-term decrease in the content of subunit p65 in CHO-K1 cells. The results suggest that nocodazole may interfere with the genome expression, and these alterations should be taken into consideration, whatever experimental studies this substance is used in.
Authors:
T V Ivanova; V N Ivanov; E S Nadezhdina
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Membrane & cell biology     Volume:  14     ISSN:  1023-6597     ISO Abbreviation:  Membr Cell Biol     Publication Date:  2001  
Date Detail:
Created Date:  2002-01-30     Completed Date:  2002-06-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9517472     Medline TA:  Membr Cell Biol     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  727-41     Citation Subset:  IM    
Affiliation:
Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region.
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MeSH Terms
Descriptor/Qualifier:
Animals
CHO Cells
Cricetinae
Fluorescent Antibody Technique
Gene Expression / drug effects
Hela Cells
Humans
NF-kappa B / drug effects*,  metabolism
Nocodazole / pharmacology*
Proto-Oncogene Proteins c-fos / drug effects*,  metabolism
Transcription Factor AP-1 / drug effects*,  metabolism
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/NF-kappa B; 0/Proto-Oncogene Proteins c-fos; 0/Transcription Factor AP-1; 31430-18-9/Nocodazole

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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