| Transcription factor AP-2β regulates the neurotransmitter phenotype and maturation of chromaffin cells. | |
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MedLine Citation:
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PMID: 20875861 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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During development, sympathetic neurons and chromaffin cells originate from bipotential sympathoadrenal (SA) progenitors arising from neural crests (NC) in the trunk regions. Recently, we showed that AP-2β, a member of the AP2 family, plays a critical role in the development of sympathetic neurons and locus coeruleus and their norepinephrine (NE) neurotransmitter phenotype. In the present study, we investigated the potential role of AP-2β in the development of NC-derived neuroendocrine chromaffin cells of the adrenal medulla and the epinephrine (EPI) phenotype determination. In support of its role in chromaffin cell development, AP-2β is prominently expressed in both embryonic and adult adrenal medulla. In adrenal chromaffin cells of the AP-2β(-/-) mouse, the expression levels of catecholamine biosynthesizing enzymes, dopamine β-hydroxylase (DBH) and phenylethanolamine-N-methyl-transferase (PNMT), as well as the SA-specific transcription factor, Phox2b, are significantly reduced compared to wild type. In addition, ultrastructural analysis demonstrated that the formation of large secretory vesicles, a hallmark of differentiated chromaffin cells, is defective in AP-2β(-/-) mice. Furthermore, the level of EPI content is largely diminished (>80%) in the adrenal gland of AP-2β(-/-) mice. Chromatin immunoprecipitation (ChIP) assays of rat adrenal gland showed that AP-2β binds to the upstream promoter of the PNMT gene in vivo; strongly suggesting that it is a direct target gene. Overall, our data suggest that AP-2β plays critical roles in the epinephrine phenotype and maturation of adrenal chromaffin cells. |
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Authors:
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Seok Jong Hong; Yang Hoon Huh; Amanda Leung; Hyun Jin Choi; Yunmin Ding; Un Jung Kang; Seung Hyun Yoo; Reinhard Buettner; Kwang-Soo Kim |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-09-27 |
Journal Detail:
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Title: Molecular and cellular neurosciences Volume: 46 ISSN: 1095-9327 ISO Abbreviation: Mol. Cell. Neurosci. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-01-10 Completed Date: 2011-07-05 Revised Date: 2012-05-15 |
Medline Journal Info:
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Nlm Unique ID: 9100095 Medline TA: Mol Cell Neurosci Country: United States |
Other Details:
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Languages: eng Pagination: 245-51 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Molecular Neurobiology Laboratory, McLean Hospital, Harvard Medical School, 115 Mill Street, Belmont, MA 02478, USA. seok-hong@northwestern.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adrenal Glands
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cytology,
metabolism Adrenergic Fibers / metabolism Animals Chromaffin Cells / cytology, physiology* Epinephrine / metabolism Female Gene Expression Mice Mice, Knockout Neurotransmitter Agents / metabolism* Norepinephrine / metabolism Phenotype* Phenylethanolamine N-Methyltransferase / genetics, metabolism Promoter Regions, Genetic Rats Secretory Vesicles / ultrastructure Transcription Factor AP-2 / genetics, metabolism* Tyrosine 3-Monooxygenase / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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MH087903/MH/NIMH NIH HHS; MH48866/MH/NIMH NIH HHS; R01 MH048866-21/MH/NIMH NIH HHS; R01 NS053919-04/NS/NINDS NIH HHS; R21 MH087903-02/MH/NIMH NIH HHS; R33 MH087903/MH/NIMH NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Neurotransmitter Agents; 0/Transcription Factor AP-2; 51-41-2/Norepinephrine; 51-43-4/Epinephrine; EC 1.14.16.2/Tyrosine 3-Monooxygenase; EC 2.1.1.28/Phenylethanolamine N-Methyltransferase |
| Comments/Corrections | |
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