Document Detail


Transcription and demethylation of TCR beta gene initiate prior to the gene rearrangement in c-kit+ thymocytes with CD3 expression: evidence of T cell commitment in the thymus.
MedLine Citation:
PMID:  8921426     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to determine whether the cells immigrating into the thymus have been committed to the T cell lineage, we examined the gene expression of the TCR complex in fetal thymus (FT) and fetal liver (FL) precursors. We previously showed that c-kit bright-positive, Pgp-1 bright-positive and lineage markers negative fetal thymocytes (c-kit+ FT cells) are the most immature cells which do not undergo gene rearrangement of the TCR beta chain. In this study, we demonstrated that the gene rearrangement of TCR gamma as well as beta chains does not occur in c-kit+ FT cells, but that the germline transcript of their TCR beta was found in J-C regions. The TCR beta gene was demethylated in c-kit+ FT cells. CD3 gamma, delta and epsilon subunit genes were also expressed at the mRNA levels in c-kit+ FT cells, but cytoplasmic protein staining divided them into two populations: cytoplasmic CD3 epsilon positive and negative cells. These features were not observed in c-kit+ FL cells. Moreover, Ly-1 expression was found on c-kit+ FT cells but not on c-kit+ FL cells. These results indicate that DNA alteration on the TCR beta gene initiates with other phenotype expression determining the T cell lineage in the thymus prior to TCR gene rearrangement.
Authors:
K Hozumi; A Kobori; T Sato; T Nishimura; S Habu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International immunology     Volume:  8     ISSN:  0953-8178     ISO Abbreviation:  Int. Immunol.     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1997-02-10     Completed Date:  1997-02-10     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8916182     Medline TA:  Int Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1473-81     Citation Subset:  IM    
Affiliation:
Department of Immunology, Tokai University School of Medicine, Isehara, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD3 / analysis*,  biosynthesis
Cell Differentiation / immunology
DNA Methylation*
Female
Gene Rearrangement, T-Lymphocyte / immunology*
Mice
Mice, Inbred C57BL
Pregnancy
Proto-Oncogene Proteins c-kit / analysis*,  biosynthesis
Receptors, Antigen, T-Cell, alpha-beta / genetics*,  metabolism*
T-Lymphocytes / immunology*,  metabolism
Thymus Gland / cytology,  immunology*
Transcription, Genetic / genetics*
Chemical
Reg. No./Substance:
0/Antigens, CD3; 0/Receptors, Antigen, T-Cell, alpha-beta; EC 2.7.10.1/Proto-Oncogene Proteins c-kit

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