Document Detail

Transcription by RNA polymerase II during Acanthamoeba differentiation.
MedLine Citation:
PMID:  9878798     Owner:  NLM     Status:  MEDLINE    
The rates of transcription of several protein coding genes during Acanthamoeba differentiation have been examined by nuclear run-on and RNase protection assays. During early encystment, transcription by RNA polymerase II increases approximately 4-fold, whereas transcription by RNA polymerases I and III is decreased, as previously described. The rates of transcription from a wide variety of individual genes are only slightly affected during the first 16 h of encystment, although profilin gene expression is markedly increased. The levels of mRNAs encoding TPBF, TATA binding protein, cyclin-dependent kinase, protein disulfide isomerase, profilin, myosin II heavy chain, ubiquitin and extendin are stable during mature cyst formation, whereas mRNAs encoding actin, S-adenosyl methionine synthase and tubulin are substantially decreased in abundance within 16 h of starvation-induced encystment. We conclude that in contrast to the negative regulation of large rRNA and 5S rRNA synthesis during differentiation, the RNA polymerase II transcription apparatus is not negatively regulated. Control of Acanthamoeba differentiation is likely to be mediated by positive regulation of genes necessary for cyst maturation.
T Orfeo; E Bateman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1443     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1998 Dec 
Date Detail:
Created Date:  1999-02-01     Completed Date:  1999-02-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  297-304     Citation Subset:  IM    
Department of Microbiology and Molecular Genetics, Markey Center for Molecular Genetics, University of Vermont, Burlington, VT 05405, USA.
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MeSH Terms
Acanthamoeba / cytology,  genetics*,  growth & development
Cell Differentiation / genetics
Gene Expression Regulation, Developmental
Genes, Protozoan / genetics
Protozoan Proteins / genetics
RNA Polymerase II*
RNA, Messenger / genetics,  metabolism
RNA, Protozoan / genetics
Transcription, Genetic
Reg. No./Substance:
0/Protozoan Proteins; 0/RNA, Messenger; 0/RNA, Protozoan; EC 2.7.7.-/RNA Polymerase II

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