| Transcription of the beta-galactoside alpha2,6-sialyltransferase gene (SIAT1) in B-lymphocytes: cell type-specific expression correlates with presence of the divergent 5'-untranslated sequence. | |
| | |
MedLine Citation:
|
PMID: 10460832 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A single gene, SIAT1, encodes ST6Gal I, the sialyltransferase that mediates transfer of alpha2,6-linked sialic acids to Galbeta1, 4GlcNAc termini of N-linked glycoproteins. In vivo, multiple SIAT1 mRNA forms, differing only in the 5'-untranslated region, are expressed in a tissue-specific manner. This mRNA heterogeneity has been attributed, at least in part, to transcription from a number of physically distinct promoter regions. In mature B-lymphocytes, SIAT1 transcription initiates at P2, a regulatory region known to function only in B-lineage cells. Bacterial chloramphenicol acetyltransferase (CAT) under the control of the P2 region encompassing 415 bp 5'- and 125 bp 3' of the transcriptional initiation site is efficiently expressed in Louckes, a mature B-lymphoblastoid cell line. In contrast, CAT expression in Reh, a T-null/B-null precursor line, and in HepG2, a hepatoma line, are 14-fold and >25-fold less than in Louckes, respectively. The data is consistent with the presence of cis -acting regulatory elements residing both 5' and 3' of the P2 transcriptional initiation site. At least 370 bp of 5'-flanking sequence, coinciding with the inclusion of AP2 and NF-kappaB sites, is necessary for high level expression in Louckes. Exon sequences 3' of the transcription start site are also important for expression. A segment from(+)32 to(+)125 (position(+)1 is transcription start site) is capable of exerting promoter-like activity in Louckes, but not in Reh or HepG2. CAT expression by P2 is negligible in Reh cells. However, enhanced CAT activity is not accompanied by elevated mRNA levels. This observation is consistent with the relief of translational restraints imposed by the(+)32 to(+)125 region. Together, the data demonstrate that efficient and cell-specific transcription regulation in mature B lymphocytes is contained in a 495 bp P2 segment that is comprised of 370 bp of 5'-flanking region and 125 bp of transcribed region of Exon X. |
| | |
Authors:
|
N W Lo; J T Lau |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Glycobiology Volume: 9 ISSN: 0959-6658 ISO Abbreviation: Glycobiology Publication Date: 1999 Sep |
Date Detail:
|
Created Date: 1999-11-04 Completed Date: 1999-11-04 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 9104124 Medline TA: Glycobiology Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 907-14 Citation Subset: IM |
Affiliation:
|
Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY 14263, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
5' Untranslated Regions* B-Lymphocytes / enzymology* Cells, Cultured Exons Gene Expression Regulation, Enzymologic* Genes, Reporter Humans Promoter Regions, Genetic Recombinant Proteins / biosynthesis Sialyltransferases / biosynthesis, genetics* Tissue Distribution Transcription, Genetic |
| Grant Support | |
ID/Acronym/Agency:
|
CA16056-21/CA/NCI NIH HHS; GM38193/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/5' Untranslated Regions; 0/Recombinant Proteins; EC 2.4.99.-/Sialyltransferases; EC 2.4.99.1/beta-D-galactoside alpha 2-6-sialyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: N-glycosylation of the carcinoembryonic antigen related cell adhesion molecule, C-CAM, from rat live...
Next Document: Characterization of human vascular endothelial cadherin glycans.