Document Detail

Transcription and RNA processing during expression of genes preceding DNA ligase gene 30 in T4-related bacteriophages.
MedLine Citation:
PMID:  16225899     Owner:  NLM     Status:  MEDLINE    
Early gene expression in bacteriophage T4 is controlled primarily by the unique early promoters, while T4-encoded RegB endoribonuclease promotes degradation of many early messages contributing to the rapid shift of gene expression from the early to middle stages. The regulatory region for the genes clustered upstream of DNA ligase gene 30 of T4 was known to carry two strong early promoters and two putative RegB sites. Here, we present the comparative analysis of the regulatory events in this region of 16 T4-type bacteriophages. The regulatory elements for control of this gene cluster, such as rho-independent terminator, at least one early promoter, the sequence for stem-loop structure, and the RegB cleavage sites have been found to be conserved in the phages studied. Also, we present experimental evidence that the initial cleavage by RegB of phages TuIa and RB69 enables degradation of early phage mRNAs by the major Escherichia coli endoribonuclease, RNase E.
Lidija Truncaite; Aurelija Zajanckauskaite; Aivaras Arlauskas; Rimas Nivinskas
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-10-12
Journal Detail:
Title:  Virology     Volume:  344     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2006-01-09     Completed Date:  2006-03-02     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  378-90     Citation Subset:  IM    
Department of Gene Engineering, Institute of Biochemistry, Mokslininku 12, 08662 Vilnius, Lithuania.
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MeSH Terms
Bacteriophage T4 / classification*,  genetics*
Base Sequence
DNA Ligases / genetics*
Gene Expression Regulation, Viral*
Genes, Viral / genetics
Molecular Sequence Data
Promoter Regions, Genetic
RNA Processing, Post-Transcriptional*
RNA, Viral / genetics,  metabolism*
Transcription, Genetic*
Reg. No./Substance:
0/RNA, Viral; EC 6.5.1.-/DNA Ligases

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