Document Detail

Transcription of HERV-E and HERV-E-related sequences in malignant and non-malignant human haematopoietic cells.
MedLine Citation:
PMID:  18929381     Owner:  NLM     Status:  MEDLINE    
Infection of a human T-cell leukaemia cell line (HSB-2) with HHV-6 led to the induction of exosome-like-particles attached to newly formed HHV-6 enveloped particles and to amplification of a 1642 bp molecule consisting of a partial human endogenous retrovirus (HERV)-E polymerase gene and repetitive sequences. We initiated an analysis of transcriptional patterns of predicted genes from HERV-E sequences in normal and malignant haematopoietic cells. Transcription patterns of regions corresponding to gag, pol and env genes at different chromosomal loci varied among cell types tested. Several specific transcripts were only observed in malignant haematopoietic cells and transcriptional activity varied among different malignant cell types. A transcript of 7.1 kb spanning the complete gag, pol and env gene region, originating from chromosome 8p23, was identified in normal peripheral blood cells and cells of the chronic myeloid leukaemia cell line K562. Our study describes new active HERV-E sequences and new loci throughout the human genome.
Bhupesh K Prusty; Harald zur Hausen; Rainer Schmidt; Romana Kimmel; Ethel-Michele de Villiers
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-10-16
Journal Detail:
Title:  Virology     Volume:  382     ISSN:  1096-0341     ISO Abbreviation:  Virology     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-11-10     Completed Date:  2008-12-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  37-45     Citation Subset:  IM    
Division for Characterization of Tumorviruses, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 242, 69120 Heidelberg, Germany.
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MeSH Terms
Blood Cells / virology
Cell Line
Cell Line, Tumor
Endogenous Retroviruses / genetics*
Gene Expression Profiling*
Genes, Viral
Leukemia, Myelogenous, Chronic, BCR-ABL Positive / virology
Transcription, Genetic*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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