Document Detail


Transcobalamin C776G genotype modifies the association between vitamin B12 and homocysteine in older Hispanics.
MedLine Citation:
PMID:  20216556     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND/OBJECTIVES: A common polymorphism, C776G, in the plasma B12 transport protein transcobalamin (TC), encodes for either proline or arginine at codon 259. This polymorphism may affect the affinity of TC for B12 and subsequent delivery of B12 to tissues.
SUBJECTS/METHODS: TC genotype and its associations with indicators of B12 status, including total B12, holotranscobalamin (holoTC), methylmalonic acid and homocysteine, were evaluated in a cohort of elderly Latinos (N=554, age 60-93 years) from the Sacramento Area Latino Study on Aging (SALSA).
RESULTS: The distribution of TC genotypes was 41.3% homozygous reference (776CC) and 11.6% homozygous variant (776GG). No differences between the homozygous genotypes were observed in total B12, holoTC, methylmalonic acid or homocysteine. The holoTC/total B12 ratio was lower in the 776GG group compared with the 776CC group (P=0.04). Significant interactions of TC genotype with total B12 (P=0.04) and with holoTC (P< or =0.03) were observed such that mean homocysteine concentrations and the odds ratios for hyperhomocysteinemia (>13 micromol/l) were higher in the 776CC subjects compared with all carriers of the G allele (776CG and 776GG combined) when total B12 (<156 pmol/l) or holoTC (<35 pmol/l) were low.
CONCLUSIONS: This population of older Latinos has a lower prevalence of the TC 776GG variant than reported for Caucasian populations. The association between vitamin B12 and homocysteine concentrations is modified by TC 776 genotype. It remains to be determined whether the TC C776G polymorphism has a significant effect on the hematological and neurological manifestations of B12 deficiency or on vascular and other morbidities associated with hyperhomocysteinemia.
Authors:
M G Garrod; L H Allen; M N Haan; R Green; J W Miller
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-03-10
Journal Detail:
Title:  European journal of clinical nutrition     Volume:  64     ISSN:  1476-5640     ISO Abbreviation:  Eur J Clin Nutr     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-05     Completed Date:  2010-10-12     Revised Date:  2011-08-01    
Medline Journal Info:
Nlm Unique ID:  8804070     Medline TA:  Eur J Clin Nutr     Country:  England    
Other Details:
Languages:  eng     Pagination:  503-9     Citation Subset:  IM    
Affiliation:
USDA, ARS Western Human Nutrition Research Center, Davis, CA 95817, USA.
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MeSH Terms
Descriptor/Qualifier:
Aged
Aging
Analysis of Variance
California
European Continental Ancestry Group
Female
Genotype
Health Surveys
Hispanic Americans / genetics*
Homocysteine / blood*
Homozygote
Humans
Hyperhomocysteinemia / genetics*
Logistic Models
Male
Methylmalonic Acid / blood
Middle Aged
Polymorphism, Single Nucleotide*
Transcobalamins / genetics*
Vitamin B 12 / blood*
Vitamin B Complex / blood
Grant Support
ID/Acronym/Agency:
AG12975/AG/NIA NIH HHS; R01 AG012975-03/AG/NIA NIH HHS; R01 AG012975-03S1/AG/NIA NIH HHS; R01 AG012975-04/AG/NIA NIH HHS; R01 AG012975-05/AG/NIA NIH HHS; R01 AG012975-06/AG/NIA NIH HHS; R01 AG012975-06S1/AG/NIA NIH HHS; R01 AG012975-07A2/AG/NIA NIH HHS; R01 AG012975-08/AG/NIA NIH HHS; R01 AG012975-09/AG/NIA NIH HHS; R01 AG012975-10/AG/NIA NIH HHS; R01 AG012975-11/AG/NIA NIH HHS; R01 AG012975-12A2/AG/NIA NIH HHS; R01 AG012975-13/AG/NIA NIH HHS; R01 AG012975-14/AG/NIA NIH HHS; R01 DK060753-01/DK/NIDDK NIH HHS; R01 DK060753-02/DK/NIDDK NIH HHS; R01 DK060753-03/DK/NIDDK NIH HHS; R03 AG033751-01A2/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
0/Transcobalamins; 12001-76-2/Vitamin B Complex; 454-28-4/Homocysteine; 516-05-2/Methylmalonic Acid; 68-19-9/Vitamin B 12

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