Document Detail

Transactivation of vimentin by beta-catenin in human breast cancer cells.
MedLine Citation:
PMID:  12750294     Owner:  NLM     Status:  MEDLINE    
The cytoplasmic and nuclear redistribution of beta-catenin and the de novo expression of vimentin are frequently involved in the epithelial-to-mesenchymal transition associated with increased invasive/migratory properties of epithelial cells. Because beta-catenin can act as a coactivator of transcription through its binding to the T-cell factor (TCF)/lymphoid enhancer factor 1 transcription factor family, we have explored the possibility that beta-catenin/TCF could directly transactivate vimentin. We first compared vimentin expression in relation with the localization of beta-catenin in eight breast cancer cell lines displaying various degrees of invasiveness and in a model of cell migration using human mammary MCF10A cells. We could thus show a cytoplasmic and/or nuclear distribution of beta-catenin in invasive/migratory cells expressing vimentin, but not in noninvasive/stationary vimentin-negative cell lines. In addition, the human vimentin promoter was found to be up-regulated by beta-catenin and TCF-4 cotransfection. Varying with the cellular background, a diminution of this up-regulation was observed when the putative beta-catenin/TCF binding site of the vimentin promoter was mutated. Our results therefore demonstrate that the vimentin promoter is a target of the beta-catenin/TCF pathway and strongly suggest an implication of this regulation in epithelial cell migration/invasion.
Christine Gilles; Myriam Polette; Mélanie Mestdagt; Béatrice Nawrocki-Raby; Philippe Ruggeri; Philippe Birembaut; Jean-Michel Foidart
Related Documents :
12415564 - Effect of 4-hydroxynonenal, a lipid peroxidation product, on exocytosis in hl-60 cells.
8547184 - 17 beta-hydroxysteroid dehydrogenase activity in endometrial cancer cells: different me...
12193564 - Functional maturation of fetal porcine beta-cells by glucagon-like peptide 1 and cholec...
67034 - Identification of beta-cells in dissociated rat pancreatic cell suspensions.
11403994 - Enzyme histochemical profile of immunohistochemically identified renshaw cells in rat l...
23894604 - Human coronavirus hku1 infection of primary human type ii alveolar epithelial cells: cy...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer research     Volume:  63     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-05-16     Completed Date:  2003-06-23     Revised Date:  2009-10-27    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2658-64     Citation Subset:  IM    
Laboratory of Tumor and Developmental Biology, University of Liège, CHU Sart-Tilman, B23, B-4000 Liège, Belgium.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Breast Neoplasms / genetics,  metabolism*
Cell Nucleus / metabolism
Cytoplasm / metabolism
Cytoskeletal Proteins / genetics,  metabolism*
DNA-Binding Proteins / genetics,  metabolism
Gene Expression Regulation, Neoplastic
Promoter Regions, Genetic
TCF Transcription Factors
Trans-Activators / genetics,  metabolism*
Transcription Factors / genetics,  metabolism
Transcriptional Activation
Tumor Cells, Cultured
Vimentin / biosynthesis*,  genetics
beta Catenin
Reg. No./Substance:
0/CTNNB1 protein, human; 0/Cytoskeletal Proteins; 0/DNA-Binding Proteins; 0/TCF Transcription Factors; 0/Tcf7L2 transcription factor; 0/Trans-Activators; 0/Transcription Factors; 0/Vimentin; 0/beta Catenin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Molecular profiling of pancreatic adenocarcinoma and chronic pancreatitis identifies multiple genes ...
Next Document:  EWI2/PGRL associates with the metastasis suppressor KAI1/CD82 and inhibits the migration of prostate...