| Transactivation of vimentin by beta-catenin in human breast cancer cells. | |
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MedLine Citation:
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PMID: 12750294 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The cytoplasmic and nuclear redistribution of beta-catenin and the de novo expression of vimentin are frequently involved in the epithelial-to-mesenchymal transition associated with increased invasive/migratory properties of epithelial cells. Because beta-catenin can act as a coactivator of transcription through its binding to the T-cell factor (TCF)/lymphoid enhancer factor 1 transcription factor family, we have explored the possibility that beta-catenin/TCF could directly transactivate vimentin. We first compared vimentin expression in relation with the localization of beta-catenin in eight breast cancer cell lines displaying various degrees of invasiveness and in a model of cell migration using human mammary MCF10A cells. We could thus show a cytoplasmic and/or nuclear distribution of beta-catenin in invasive/migratory cells expressing vimentin, but not in noninvasive/stationary vimentin-negative cell lines. In addition, the human vimentin promoter was found to be up-regulated by beta-catenin and TCF-4 cotransfection. Varying with the cellular background, a diminution of this up-regulation was observed when the putative beta-catenin/TCF binding site of the vimentin promoter was mutated. Our results therefore demonstrate that the vimentin promoter is a target of the beta-catenin/TCF pathway and strongly suggest an implication of this regulation in epithelial cell migration/invasion. |
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Authors:
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Christine Gilles; Myriam Polette; Mélanie Mestdagt; Béatrice Nawrocki-Raby; Philippe Ruggeri; Philippe Birembaut; Jean-Michel Foidart |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cancer research Volume: 63 ISSN: 0008-5472 ISO Abbreviation: Cancer Res. Publication Date: 2003 May |
Date Detail:
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Created Date: 2003-05-16 Completed Date: 2003-06-23 Revised Date: 2009-10-27 |
Medline Journal Info:
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Nlm Unique ID: 2984705R Medline TA: Cancer Res Country: United States |
Other Details:
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Languages: eng Pagination: 2658-64 Citation Subset: IM |
Affiliation:
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Laboratory of Tumor and Developmental Biology, University of Liège, CHU Sart-Tilman, B23, B-4000 Liège, Belgium. cgilles@ulg.ac.be |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Breast Neoplasms
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genetics,
metabolism* Cell Nucleus / metabolism Cytoplasm / metabolism Cytoskeletal Proteins / genetics, metabolism* DNA-Binding Proteins / genetics, metabolism Gene Expression Regulation, Neoplastic Humans Promoter Regions, Genetic TCF Transcription Factors Trans-Activators / genetics, metabolism* Transcription Factors / genetics, metabolism Transcriptional Activation Transfection Tumor Cells, Cultured Vimentin / biosynthesis*, genetics beta Catenin |
| Chemical | |
Reg. No./Substance:
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0/CTNNB1 protein, human; 0/Cytoskeletal Proteins; 0/DNA-Binding Proteins; 0/TCF Transcription Factors; 0/Tcf7L2 transcription factor; 0/Trans-Activators; 0/Transcription Factors; 0/Vimentin; 0/beta Catenin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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