Document Detail


Transactivation of vimentin by beta-catenin in human breast cancer cells.
MedLine Citation:
PMID:  12750294     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cytoplasmic and nuclear redistribution of beta-catenin and the de novo expression of vimentin are frequently involved in the epithelial-to-mesenchymal transition associated with increased invasive/migratory properties of epithelial cells. Because beta-catenin can act as a coactivator of transcription through its binding to the T-cell factor (TCF)/lymphoid enhancer factor 1 transcription factor family, we have explored the possibility that beta-catenin/TCF could directly transactivate vimentin. We first compared vimentin expression in relation with the localization of beta-catenin in eight breast cancer cell lines displaying various degrees of invasiveness and in a model of cell migration using human mammary MCF10A cells. We could thus show a cytoplasmic and/or nuclear distribution of beta-catenin in invasive/migratory cells expressing vimentin, but not in noninvasive/stationary vimentin-negative cell lines. In addition, the human vimentin promoter was found to be up-regulated by beta-catenin and TCF-4 cotransfection. Varying with the cellular background, a diminution of this up-regulation was observed when the putative beta-catenin/TCF binding site of the vimentin promoter was mutated. Our results therefore demonstrate that the vimentin promoter is a target of the beta-catenin/TCF pathway and strongly suggest an implication of this regulation in epithelial cell migration/invasion.
Authors:
Christine Gilles; Myriam Polette; Mélanie Mestdagt; Béatrice Nawrocki-Raby; Philippe Ruggeri; Philippe Birembaut; Jean-Michel Foidart
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer research     Volume:  63     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-05-16     Completed Date:  2003-06-23     Revised Date:  2009-10-27    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2658-64     Citation Subset:  IM    
Affiliation:
Laboratory of Tumor and Developmental Biology, University of Liège, CHU Sart-Tilman, B23, B-4000 Liège, Belgium. cgilles@ulg.ac.be
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MeSH Terms
Descriptor/Qualifier:
Breast Neoplasms / genetics,  metabolism*
Cell Nucleus / metabolism
Cytoplasm / metabolism
Cytoskeletal Proteins / genetics,  metabolism*
DNA-Binding Proteins / genetics,  metabolism
Gene Expression Regulation, Neoplastic
Humans
Promoter Regions, Genetic
TCF Transcription Factors
Trans-Activators / genetics,  metabolism*
Transcription Factors / genetics,  metabolism
Transcriptional Activation
Transfection
Tumor Cells, Cultured
Vimentin / biosynthesis*,  genetics
beta Catenin
Chemical
Reg. No./Substance:
0/CTNNB1 protein, human; 0/Cytoskeletal Proteins; 0/DNA-Binding Proteins; 0/TCF Transcription Factors; 0/Tcf7L2 transcription factor; 0/Trans-Activators; 0/Transcription Factors; 0/Vimentin; 0/beta Catenin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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