Document Detail


Transactivation of cytosolic alanine aminotransferase gene promoter by p300 and c-Myb.
MedLine Citation:
PMID:  20573775     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Alanine aminotransferase (Alt) provides a molecular link between carbohydrate and amino acid metabolism. In the cell context, the predominant Alt isozyme is located in the cytosol. To gain insight into the transcriptional regulation of the cytosolic alt gene (calt), we cloned and characterized the calt promoter from gilthead sea bream (Sparus aurata). Transient transfection of sea bass larvae cells with deleted calt promoter constructs and electrophoretic mobility shift assays allowed us to identify p300 and c-Myb as new factors in the transcriptional regulation of calt expression. Transfection studies carried out with an acetylase-deficient mutant p300 (p300DY) revealed that the acetyltransferase activity of p300 is essential for the p300-mediated transcriptional activation of S. aurata calt. We had previously found up-regulation of liver cAlt2, an alternatively spliced isoform of calt, under gluconeogenic conditions and in streptozotocin (STZ)-treated S. aurata. Quantitative RT-PCR assays showed that increased p300 and c-Myb mRNA levels in the liver of starved S. aurata contribute to enhancing the transcription of cAlt2. Consistently, the administration of insulin decreased both p300 and c-Myb expression. The mRNA levels of p300 and c-Myb were also analyzed in the liver of STZ-induced diabetic S. aurata. Treatment with STZ increased the expression of p300, whereas it decreased c-Myb. Our findings suggest an involvement of p300 and c-Myb in up-regulation of cAlt2 in the liver of S. aurata under starvation. In addition, these results provide evidence for a role of p300 in diabetes.
Authors:
Ida G Anemaet; Juan Diego González; María C Salgado; Marina Giralt; Felipe Fernández; Isabel V Baanante; Isidoro Metón
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-23
Journal Detail:
Title:  Journal of molecular endocrinology     Volume:  45     ISSN:  1479-6813     ISO Abbreviation:  J. Mol. Endocrinol.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-13     Completed Date:  2010-12-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8902617     Medline TA:  J Mol Endocrinol     Country:  England    
Other Details:
Languages:  eng     Pagination:  119-32     Citation Subset:  IM    
Affiliation:
Departament de Bioquímica i Biologia Molecular, Facultat de Farmàcia, Universitat de Barcelona, Joan XXIII s/n, 08028 Barcelona, Spain.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/DQ114991
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MeSH Terms
Descriptor/Qualifier:
5' Flanking Region / genetics
Alanine Transaminase / genetics*
Animals
Base Sequence
Bass
Cloning, Molecular
Consensus Sequence / genetics
Cytosol / drug effects,  enzymology*
Gene Expression Regulation, Enzymologic / drug effects
Histone Acetyltransferases
Insulin / pharmacology
Liver / metabolism
Molecular Sequence Data
Multiprotein Complexes / metabolism
Mutation / genetics
Promoter Regions, Genetic*
Protein Binding / drug effects
Proto-Oncogene Proteins c-myb / metabolism*
Response Elements / genetics
Sea Bream / genetics*
Sequence Deletion
Transcriptional Activation / genetics*
p300-CBP Transcription Factors / metabolism*
Chemical
Reg. No./Substance:
0/Multiprotein Complexes; 0/Proto-Oncogene Proteins c-myb; 11061-68-0/Insulin; EC 2.3.1.48/Histone Acetyltransferases; EC 2.3.1.48/p300-CBP Transcription Factors; EC 2.6.1.2/Alanine Transaminase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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