Document Detail

Trans-epithelial transport of the betalain pigments indicaxanthin and betanin across Caco-2 cell monolayers and influence of food matrix.
MedLine Citation:
PMID:  22806766     Owner:  NLM     Status:  Publisher    
PURPOSE: This study investigated the absorption mechanism of the phytochemicals indicaxanthin and betanin and the influence of their food matrix (cactus pear and red beet) on the intestinal transport. METHODS: Trans-epithelial transport of dietary-consistent amounts of indicaxanthin and betanin in Caco-2 cell monolayers seeded on Transwell(R) inserts was measured in apical to basolateral (AP-BL) and basolateral to apical (BL-AP) direction, under an inwardly directed pH gradient (pH 6.0/7.4, AP/BL) mimicking luminal and serosal sides of human intestinal epithelium. The effect of inhibitors of membrane transporters on the absorption was also evaluated. Contribution of the paracellular route was investigated after EDTA treatment of the cell monolayer. In vitro digestion of betalainic food was performed to provide a post-intestinal fraction containing bioaccessible pigments. RESULTS: Apparent permeability coefficients (P (app)) in the absorptive direction were (4.4 ± 0.4) × 10(-6) and (3.2 ± 0.3) × 10(-6) cm s(-1) for indicaxanthin and betanin, respectively. Transport of indicaxanthin was non-polarized, linear as a function of time and concentration, and unaffected by inhibitors of membrane transporters. Betanin exhibited significantly different bidirectional P (app) values and non-linear efflux kinetics. The concentration-dependent betanin efflux was described by a kinetic model including one non-saturable (K ( d ) = 0.042 μL cm(-2) min(-1)) and one saturable component identified as the apical multidrug resistance-associated protein 2 (MRP2; K ( m ) = 275 μM; J (max) = 42 pmol min(-1) cm(-2)). Permeation of both betalains increased remarkably after EDTA treatment of the cell monolayer. Neither indicaxanthin nor betanin underwent metabolic transformation. Food matrix did not affect trans-epithelial transfer of indicaxanthin, but reduced the absorption rate of betanin, red beet more than cactus pear. CONCLUSIONS: Dietary indicaxanthin and betanin can substantially be absorbed through paracellular junctions of intestinal epithelial cells. Additional trans-membrane permeation can be considered for betanin, whose absorption is limited by a MRP2-mediated efflux and negatively affected by its food matrix. Present findings are consistent with the quite higher bioavailability of indicaxanthin over betanin established in humans.
L Tesoriere; C Gentile; F Angileri; A Attanzio; M Tutone; M Allegra; M A Livrea
Related Documents :
23062966 - Rapidly rising food prices and the experience of food insecurity in urban ethiopia: imp...
15870376 - Mass outbreak of food poisoning disease caused by small amounts of staphylococcal enter...
24993286 - Urban food insecurity in the context of high food prices: a community based cross secti...
6205766 - The histone h5 variant in xenopus laevis.
18552296 - Modulation, individual variation and the role of lingual sensory afferents in the contr...
2782866 - Responses of lean and obese subjects to preloads, deprivation, and palatability.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-18
Journal Detail:
Title:  European journal of nutrition     Volume:  -     ISSN:  1436-6215     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888704     Medline TA:  Eur J Nutr     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Dipartimento di Scienze e Tecnologie Molecolari e Biomolecolari (STEMBIO), Università di Palermo, Via Michele Cipolla 74, 90123, Palermo, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Octreotide alleviates obesity by reducing intestinal glucose absorption and inhibiting low-grade inf...
Next Document:  Is DSM-IV criterion A2 associated with PTSD diagnosis and symptom severity?