Document Detail


Trans-activation by HIV-1 Tat via a heterologous RNA binding protein.
MedLine Citation:
PMID:  2117500     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The HIV-1 trans-activator Tat increases levels of viral gene expression and replication. The target for Tat is an RNA stem-loop called TAR, located at the 5' end of all viral transcripts. To study the mechanism of action and map functional domains of Tat, we fused Tat to the coat protein of bacteriophage MS2, an RNA binding protein. TAR in the HIV-1 LTR was replaced by the operator, the RNA target of the coat protein. The hybrid Tat-coat protein trans-activated HIV-1 LTRs containing either TAR or operator sequences. Mutations in the operator that weaken binding of the coat protein in vitro led to decreased levels of trans-activation in vivo. Deletions in Tat within the hybrid Tat-coat protein identified activation and RNA binding domains of Tat. These experiments suggest that trans-activation by Tat can occur independently of TAR RNA and DNA binding proteins and that Tat exerts its effects on HIV-1 transcription by directly interacting with the TAR RNA stem-loop.
Authors:
M J Selby; B M Peterlin
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell     Volume:  62     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  1990 Aug 
Date Detail:
Created Date:  1990-09-27     Completed Date:  1990-09-27     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  769-76     Citation Subset:  IM; X    
Affiliation:
Howard Hughes Medical Institute, University of California, San Francisco 94143-0724.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
Capsid / genetics
Carrier Proteins / physiology*
Gene Expression Regulation, Viral*
Gene Products, tat / physiology*
HIV-1 / genetics*
Hela Cells
Humans
Molecular Sequence Data
Operator Regions, Genetic
RNA, Viral / genetics*
RNA-Binding Proteins
Recombinant Fusion Proteins
Regulatory Sequences, Nucleic Acid*
Structure-Activity Relationship
Trans-Activators / physiology*
Transcription, Genetic*
tat Gene Products, Human Immunodeficiency Virus
Grant Support
ID/Acronym/Agency:
AI25109/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Gene Products, tat; 0/RNA, Viral; 0/RNA-Binding Proteins; 0/Recombinant Fusion Proteins; 0/Trans-Activators; 0/tat Gene Products, Human Immunodeficiency Virus

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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