Document Detail

Trans-SILAC: sorting out the non-cell-autonomous proteome.
MedLine Citation:
PMID:  20935649     Owner:  NLM     Status:  In-Process    
Non-cell-autonomous proteins are incorporated into cells that form tight contacts or are invaded by bacteria, but identifying the full repertoire of transferred proteins has been a challenge. Here we introduce a quantitative proteomics approach to sort out non-cell-autonomous proteins synthesized by other cells or intracellular pathogens. Our approach combines stable-isotope labeling of amino acids in cell culture (SILAC), high-purity cell sorting and bioinformatics analysis to identify the repertoire of relevant non-cell-autonomous proteins. This 'trans-SILAC' method allowed us to discover many proteins transferred from human B to natural killer cells and to measure biosynthesis rates of Salmonella enterica proteins in infected human cells. Trans-SILAC should be a useful method to examine protein exchange between different cells of multicellular organisms or pathogen and host.
Oded Rechavi; Matan Kalman; Yuan Fang; Helly Vernitsky; Jasmine Jacob-Hirsch; Leonard J Foster; Yoel Kloog; Itamar Goldstein
Related Documents :
7884799 - Characteristics of invasion of hep-2 cells by providencia alcalifaciens.
6370399 - In vitro binding of candida albicans yeast cells to human fibronectin.
2405889 - Demonstration of lipid a-binding proteins on murine lymphoma cells using r-mutant gram-...
8031099 - Effect of a pseudomonas rhamnolipid biosurfactant on cell hydrophobicity and biodegrada...
9353059 - Utilization of similar mechanisms by legionella pneumophila to parasitize two evolution...
10417189 - Campylobacter jejuni 81-176 associates with microtubules and dynein during invasion of ...
24391539 - Synaptic integration by ng2 cells.
16815079 - Sftg international collaborative study on in vitro micronucleus test i. general conditi...
9816019 - Pml/raralpha, a fusion protein in acute promyelocytic leukemia, prevents growth factor ...
Publication Detail:
Type:  Journal Article     Date:  2010-10-10
Journal Detail:
Title:  Nature methods     Volume:  7     ISSN:  1548-7105     ISO Abbreviation:  Nat. Methods     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101215604     Medline TA:  Nat Methods     Country:  United States    
Other Details:
Languages:  eng     Pagination:  923-7     Citation Subset:  IM    
Department of Neurobiology, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  IL-12 initiates tumor rejection via lymphoid tissue-inducer cells bearing the natural cytotoxicity r...
Next Document:  De novo assembly and analysis of RNA-seq data.