Document Detail

Trans-SILAC: sorting out the non-cell-autonomous proteome.
MedLine Citation:
PMID:  20935649     Owner:  NLM     Status:  In-Process    
Non-cell-autonomous proteins are incorporated into cells that form tight contacts or are invaded by bacteria, but identifying the full repertoire of transferred proteins has been a challenge. Here we introduce a quantitative proteomics approach to sort out non-cell-autonomous proteins synthesized by other cells or intracellular pathogens. Our approach combines stable-isotope labeling of amino acids in cell culture (SILAC), high-purity cell sorting and bioinformatics analysis to identify the repertoire of relevant non-cell-autonomous proteins. This 'trans-SILAC' method allowed us to discover many proteins transferred from human B to natural killer cells and to measure biosynthesis rates of Salmonella enterica proteins in infected human cells. Trans-SILAC should be a useful method to examine protein exchange between different cells of multicellular organisms or pathogen and host.
Oded Rechavi; Matan Kalman; Yuan Fang; Helly Vernitsky; Jasmine Jacob-Hirsch; Leonard J Foster; Yoel Kloog; Itamar Goldstein
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Publication Detail:
Type:  Journal Article     Date:  2010-10-10
Journal Detail:
Title:  Nature methods     Volume:  7     ISSN:  1548-7105     ISO Abbreviation:  Nat. Methods     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101215604     Medline TA:  Nat Methods     Country:  United States    
Other Details:
Languages:  eng     Pagination:  923-7     Citation Subset:  IM    
Department of Neurobiology, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel.
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