Document Detail

Trans-membrane uptake and intracellular metabolism of fatty acids in Atlantic salmon (Salmo salar L.) hepatocytes.
MedLine Citation:
PMID:  20186497     Owner:  NLM     Status:  MEDLINE    
To elucidate if the trans-membrane uptake of fatty acids is protein-mediated, the uptake of oleic acid (18:1n-9), linoleic acid (18:2n-6), alpha-linolenic acid (18:3n-3), eicosapentaenoic acid (20:5n-3) and docosahexaenoic acid (22:6n-3) was investigated in vitro in Atlantic salmon (Salmo salar L.) primary hepatocytes. Firstly, optimal fatty acid incubation time and concentration were established for trans-membrane 18:n-9 uptake. Based on saturation kinetics, a 2-h incubation time and 37.5 muM were used for the following experiments. Secondly, in order to identify whether trans-membrane fatty acid uptake in hepatocytes was mainly passive or protein mediated, hepatocytes were pre-incubated with membrane protein inhibitors followed by 2 h of incubation with [1-(14)C] labelled 18:1n-9, 18:2n-6, 18:3n-3, 20:5n-3 and 22:6n-3. Fatty acid uptake into hepatocytes was highest with 20:5n-3 and 22:6n-3 and lowest with 18:1n-9. Phloretin was the most potent fatty acid uptake inhibitor, inhibiting uptake in the following order: 20:5n-3 > 18:3n-3 = 22:6n-3 > 18:2n-6 > 18:1n-9. The uptake of FA in Atlantic salmon hepatocytes seem to be due to both saturable and inhibitable protein mediated uptake, as well as passive uptake processes with more unsaturated and long fatty acids (20:n-3 > 22:6n-3 = 18:3n-3 > 18:2n-6) being more dependent on membrane protein mediated uptake compared to 18:1n-9.
Jishu Zhou; Ingunn Stubhaug; Bente E Torstensen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-02-27
Journal Detail:
Title:  Lipids     Volume:  45     ISSN:  1558-9307     ISO Abbreviation:  Lipids     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-03-25     Completed Date:  2010-06-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  301-11     Citation Subset:  IM    
College of Animal Science and Technology, Northwest A&F University, Yangling, 712100 Shaanxi, China.
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MeSH Terms
Cell Membrane / metabolism*
Cell Survival / drug effects
Fatty Acids / metabolism*,  pharmacokinetics
Fish Oils / pharmacokinetics,  pharmacology
Hepatocytes / metabolism*
Intracellular Space / metabolism*
Lipid Metabolism / physiology
Metabolism / physiology
Plant Oils / pharmacokinetics,  pharmacology
Salmo salar / metabolism*
Time Factors
alpha-Linolenic Acid / metabolism,  pharmacokinetics
Reg. No./Substance:
0/Fatty Acids; 0/Fish Oils; 0/Plant Oils; 463-40-1/alpha-Linolenic Acid

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