| Training response of mitochondrial transcription factors in human skeletal muscle. | |
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MedLine Citation:
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PMID: 19681768 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: Mitochondrial function is essential for physical performance and health. Aerobic fitness is positively associated with mitochondrial (mt) biogenesis in muscle cells through partly unknown regulatory mechanisms. The present study aimed to investigate the influence of exercise and training status on key mt transcription factors in relation to oxidative capacity in human skeletal muscle. METHODS: The basal mRNA and protein levels of mitochondrial transcription factor A (TFAM), mitochondrial transcription factors B1 (TFB1M) or B2 (TFB2M), and mRNA levels of mitochondrial transcription termination factor (mTERF), were measured in a cross-sectional study with elite athletes (EA) and moderately active (MA) and the basal mRNA levels of these factors were measured during a 10-day endurance training programme with (R-leg) and without (NR-leg) restricted blood flow to the working leg. RESULTS: TFAM protein expression was significantly higher in the EA than in the MA, while protein levels of TFB1M and TFB2M were not different between the groups. There was no difference between EA and MA, or any effect with training on TFAM mRNA levels. However, the mRNA levels of TFB1M, TFB2M and mTERF were higher in EA compared with MA. For TFB1M and TFB2M, the mRNA expression was increased in the R-leg after 10 days of training, but not in the NR-leg. mTERF mRNA levels were higher in EA compared with MA. CONCLUSION: This study further establishes that TFAM protein levels are higher in conditions with enhanced oxidative capacity. The mRNA levels of TFB1M and TFB2M are influenced by endurance training, possibly suggesting a role for these factors in the regulation of exercise-induced mitochondrial biogenesis. |
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Authors:
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J Norrbom; S E Wallman; T Gustafsson; H Rundqvist; E Jansson; C J Sundberg |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-08-13 |
Journal Detail:
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Title: Acta physiologica (Oxford, England) Volume: 198 ISSN: 1748-1716 ISO Abbreviation: Acta Physiol (Oxf) Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2010-01-06 Completed Date: 2010-03-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101262545 Medline TA: Acta Physiol (Oxf) Country: England |
Other Details:
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Languages: eng Pagination: 71-9 Citation Subset: IM |
Affiliation:
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Division of Clinical Physiology, Department of Physiology and Pharmacology, Karolinska Institutet, 171 77 Stockholm, Sweden. jessica.norrbom@ki.se |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Basic-Leucine Zipper Transcription Factors / metabolism* Blotting, Western Cross-Sectional Studies DNA-Binding Proteins / metabolism* Gene Expression Gene Expression Profiling Humans Male Mitochondria, Muscle / metabolism Mitochondrial Proteins / metabolism* Muscle, Skeletal / metabolism* Physical Fitness / physiology* RNA, Messenger Reverse Transcriptase Polymerase Chain Reaction Transcription Factors / metabolism* Young Adult |
| Chemical | |
Reg. No./Substance:
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0/Basic-Leucine Zipper Transcription Factors; 0/DNA-Binding Proteins; 0/MTERF protein, human; 0/Mitochondrial Proteins; 0/RNA, Messenger; 0/TFAM protein, human; 0/TFB1M protein, human; 0/Transcription Factors |
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