Document Detail


Trained men display increased Basal heat shock protein content of skeletal muscle.
MedLine Citation:
PMID:  18580405     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
PURPOSE: 1) To compare the baseline levels of heat shock and antioxidant protein content in the skeletal muscle of trained and untrained humans and 2) to characterize the exercise-induced stress response of aerobically trained human skeletal muscle to an acute exercise challenge. METHODS: Resting muscle biopsies were obtained from the vastus lateralis muscle of six untrained and six aerobically trained young males. To characterize the stress response of a trained population, the trained subjects also performed a 45-min nondamaging running exercise protocol at an intensity corresponding to 75% of V O2max. Muscle biopsies were obtained from the vastus lateralis muscle at 48 h and 7 d after exercise. RESULTS: Trained subjects displayed significantly higher (P<0.05) resting levels of heat shock protein 60 (HSP60, 25%), alphaB-crystallin (43%), and manganese superoxide (MnSOD, 45%) protein content compared with untrained subjects. Trained subjects also exhibited no significant change (P > 0.05) in resting levels of HSP70 (16%), HSC70 (13%), and total superoxide dismutase (SOD) activity (46%) compared with untrained subjects. Resting HSP27 levels were unaffected by exercise training (P > 0.05). In the trained subjects, exercise failed to induce significant increases (P>0.05)in muscle content of HSP70, HSC70, HSP60, HSP27, alphaB-crystallin, and MnSOD protein content or in the activity of SOD at any time point after exercise. CONCLUSION: This study demonstrates for the first time that trained men display a selective up-regulation of basal heat shock and antioxidant protein content and do not exhibit a stress response to customary running exercise. It is suggested that an increase in these protective systems functions to maintain homeostasis during the stress of exercise by protecting against disruptions to the cytoskeleton/contractile machinery, by maintaining redox balance, and by facilitating mitochondrial biogenesis.
Authors:
James P Morton; Don P M Maclaren; Nigel T Cable; Iain T Campbell; Louise Evans; Anna C Kayani; Anne McArdle; Barry Drust
Related Documents :
19566475 - Effects of acute exercise and long-term exercise on total na+,k+ -atpase content and na...
15000895 - Assay of mitochondrial functions by resazurin in vitro.
15640275 - Exercise-induced metallothionein expression in human skeletal muscle fibres.
19681095 - Chronic exercise decreases cytokine production in healthy rat skeletal muscle.
24149395 - The effect of chinese yuanji-dance on dynamic balance and the associated attentional de...
10650995 - Plasma cortisol and beta-endorphin concentrations in trained and over-trained standardb...
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Medicine and science in sports and exercise     Volume:  40     ISSN:  0195-9131     ISO Abbreviation:  Med Sci Sports Exerc     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-06-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8005433     Medline TA:  Med Sci Sports Exerc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1255-62     Citation Subset:  IM; S    
Affiliation:
Research Institute for Sport and Exercise Sciences, Liverpool John Moores University, Liverpool, UNITED KINGDOM. J.P.Morton@ljmu.ac.uk
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Effect of hindlimb unloading on salt and water intake and output in male and female rats.
Next Document:  Low-intensity exercise exerts beneficial effects on plasma lipids via PPARgamma.