Document Detail


Trafficking of Candida albicans through oral epithelial endocytic compartments.
MedLine Citation:
PMID:  20854227     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Oral epithelial cells are the first cells that interact with C. albicans during the establishment of oropharyngeal candidiasis. Following initial adhesion, C. albicans invades oral epithelial cells by inducing its own endocytosis and gains access to epithelial vacuolar compartments. Epithelial endocytic pathways are key innate immune mechanisms in host defense. We examined the trafficking of C. albicans through oral epithelial endocytic compartments. We present evidence that C. albicans is internalized by oral epithelial cells through actin-dependent clathrin-mediated endocytosis and is taken into vacuolar compartments immediately following its internalization. C. albicans-containing endosomes transiently acquired early endosomal marker EEA1, but showed marked defects in acquisition of late endosomal marker LAMP1 and lysosomal marker cathepsin D. Defective endolysosomal maturation may partially explain the inability of oral epithelial cells to kill C. albicans.
Authors:
Xiang Ru Zhao; Cristina Cunha Villar
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-09-21
Journal Detail:
Title:  Medical mycology     Volume:  49     ISSN:  1460-2709     ISO Abbreviation:  Med. Mycol.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-17     Completed Date:  2011-04-20     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  9815835     Medline TA:  Med Mycol     Country:  England    
Other Details:
Languages:  eng     Pagination:  212-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Candida albicans / pathogenicity*
Cathepsin D / analysis
Cell Line
Endocytosis*
Endosomes / chemistry,  microbiology*
Epithelial Cells / microbiology*
Humans
Lysosome-Associated Membrane Glycoproteins / analysis
Vesicular Transport Proteins / analysis
Grant Support
ID/Acronym/Agency:
UL 1RR025767/RR/NCRR NIH HHS; UL1 RR025767/RR/NCRR NIH HHS; UL1 TR000149/TR/NCATS NIH HHS
Chemical
Reg. No./Substance:
0/LAMP1 protein, human; 0/Lysosome-Associated Membrane Glycoproteins; 0/Vesicular Transport Proteins; 0/early endosome antigen 1; EC 3.4.23.5/CTSD protein, human; EC 3.4.23.5/Cathepsin D

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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