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Traditionally used Veronica officinalis inhibits proinflammatory mediators via the NF-kB signalling pathway in a human lung cell line.
MedLine Citation:
PMID:  23142555     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
ETHNOPHARMACOLOGICAL RELEVANCE: Extracts from Veronica officinalis L. are tradionally used for the treatment of lung diseases, however the effective compounds and the mode of action are still unknown. AIM OF THE STUDY: Here we analyzed the effects of a standardized Veronica extract on genes expression and signalling proteins production associated with the development of inflammatory lung diseases. MATERIAL AND METHODS: The degranulation capacity of primary mast cells, as well as gene expression and release of inflammatory mediators from human lung epithelial cells (A549 cells) were analyzed in relation to the synthetic drugs azelastine and dexamethasone. Gene and protein expression of cyclooxygenase-2 were investigated by semi-quantitative RT-PCR and western blotting, respectively. The involvement of phosphorylated mitogen-activated protein kinases and NF-κB signaling in regulation of these molecules were characterized by western blotting and electrophoretic mobility shift assays. Characteristic extract components were identified by LC-MS and verminoside was quantified by HPLC analysis. RESULTS: We demonstrated that Veronica officinalis has small influence on the degranulation capacity of mast cells rather inhibits gene and protein expression of the chemokine eotaxin in A549 lung epithelial cells, which is essential for recruitment of inflammatory-associated cells in lung diseases. Furthermore, release of the inflammatory mediator PGE(2) was diminished through inhibition of COX-2 expression via the NF-κB signaling pathway in TNF-α-activated A549 cells. Phytochemical analysis identified verproside and verminoside as the most abundant iridoid glycosides. CONCLUSION: Our results are a contribution to explaining the observed anti-inflammatory effects of Veronica offcinalis extract on a molecular level. However, its clinical potency has at first to be proven in animals and subsequently in clinical trials.
Authors:
Carsten Gründemann; Manuel Garcia-Käufer; Barbara Sauer; Evi Stangenberg; Mathias Könczöl; Irmgard Merfort; Martin Zehl; Roman Huber
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-8
Journal Detail:
Title:  Journal of ethnopharmacology     Volume:  -     ISSN:  1872-7573     ISO Abbreviation:  J Ethnopharmacol     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7903310     Medline TA:  J Ethnopharmacol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.
Affiliation:
Center for Complementary Medicine, Department of Environmental Health Sciences, University Medical Center Freiburg, Breisacher Str. 115B, 79111 Freiburg, Germany. Electronic address: carsten.gruendemann@uniklinik-freiburg.de.
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