| Traditional Herbal Medicine, Rikkunshito, Induces HSP60 and Enhances Cytoprotection of Small Intestinal Mucosal Cells as a Nontoxic Chaperone Inducer. | |
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MedLine Citation:
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PMID: 21822440 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Increasing incidence of small intestinal ulcers associated with nonsteroidal anti-inflammatory drugs (NSAIDs) has become a topic with recent advances of endoscopic technology. However, the pathogenesis and therapy are not fully understood. The aim of this study is to examine the effect of Rikkunshito (TJ-43), a traditional herbal medicine, on expression of HSP60 and cytoprotective ability in small intestinal cell line (IEC-6). Effect of TJ-43 on HSP60 expression in IEC-6 cells was evaluated by immunoblot analysis. The effect of TJ-43 on cytoprotective abilities of IEC-6 cells against hydrogen peroxide or indomethacin was studied by MTT assay, LDH-release assay, caspase-8 activity, and TUNEL. HSP60 was significantly induced by TJ-43. Cell necrosis and apoptosis were significantly suppressed in IEC-6 cells pretreated by TJ-43 with overexpression of HSP60. Our results suggested that HSP60 induced by TJ-43 might play an important role in protecting small intestinal epithelial cells from apoptosis and necrosis in vitro. |
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Authors:
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Kumiko Tamaki; Michiro Otaka; Tomoyoshi Shibuya; Naoto Sakamoto; Soh Yamamoto; Masaru Odashima; Hideaki Itoh; Sumio Watanabe |
Publication Detail:
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Type: Journal Article Date: 2011-07-21 |
Journal Detail:
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Title: Evidence-based complementary and alternative medicine : eCAM Volume: 2012 ISSN: 1741-4288 ISO Abbreviation: Evid Based Complement Alternat Med Publication Date: 2012 |
Date Detail:
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Created Date: 2011-08-08 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101215021 Medline TA: Evid Based Complement Alternat Med Country: United States |
Other Details:
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Languages: eng Pagination: 278958 Citation Subset: - |
Affiliation:
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Department of Gastroenterology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-Ku, Tokyo 113-8421, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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