Document Detail


Tracking the pathway of calcium phosphate/DNA nanoparticles during cell transfection by incorporation of red-fluorescing tetramethylrhodamine isothiocyanate-bovine serum albumin into these nanoparticles.
MedLine Citation:
PMID:  17031704     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Calcium phosphate nanoparticles were prepared by precipitation from water and were then functionalized by DNA. These particles are taken up by living cells and function as gene transfer agents, i.e., the DNA is brought into a cell's nucleus and is incorporated there into the cell's genome (transfection). DNA which encodes for enhanced green fluorescent protein leads to green fluorescence of successfully transfected cells. By adding the red-fluorescing marker tetramethylrhodamine isothiocyanate-bovine serum albumin (TRITC-BSA) to the nanoparticles, their pathway into the cell and within the cell could be followed by fluorescence microscopy. A clear correlation between the uptake of nanoparticles and the efficiency of transfection was found. Aggregates of DNA/TRITC-BSA alone were not able to enter the cells, i.e., the inorganic nanoparticles are necessary as a carrier through the cell membrane.
Authors:
Viktoriya Sokolova; Anna Kovtun; Rolf Heumann; Matthias Epple
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Publication Detail:
Type:  Journal Article     Date:  2006-10-10
Journal Detail:
Title:  Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry     Volume:  12     ISSN:  0949-8257     ISO Abbreviation:  J. Biol. Inorg. Chem.     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-01-31     Completed Date:  2007-04-10     Revised Date:  2013-04-05    
Medline Journal Info:
Nlm Unique ID:  9616326     Medline TA:  J Biol Inorg Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  174-9     Citation Subset:  IM    
Affiliation:
Institute of Inorganic Chemistry, University of Duisburg-Essen, Universitaetsstrasse 5-7, 45117, Essen, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcium Phosphates / chemistry*
Cattle
Cell Line
DNA / administration & dosage*,  chemistry,  pharmacokinetics*
Drug Delivery Systems
Green Fluorescent Proteins / analysis
Humans
Microscopy, Fluorescence
Nanoparticles / administration & dosage,  analysis*,  chemistry
Particle Size
Rhodamines / analysis
Serum Albumin, Bovine / administration & dosage,  pharmacokinetics*
Transfection / methods*
Chemical
Reg. No./Substance:
0/Calcium Phosphates; 0/Rhodamines; 0/Serum Albumin, Bovine; 0/enhanced green fluorescent protein; 147336-22-9/Green Fluorescent Proteins; 4158-89-8/tetramethylrhodamine isothiocyanate; 9007-49-2/DNA; 97Z1WI3NDX/calcium phosphate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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